Strain Information | |
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Image | |
BRC No. | RBRC05845 |
Type | Transgene |
Species | Mus musculus |
Strain name | B6D2-Tg(hOX-Arch/EGFP)1Ahky |
Former Common name | Orexin-Arch, B6-hOX-Arch/GFP Tg, B6D2-Tg(hOX-Arch/EGFP)1Ahky |
H-2 Haplotype | |
ES Cell line | |
Background strain | Jcl:B6D2F1 |
Appearance | |
Strain development | Dr. Akihiro Yamanaka, National Institute for Physiological Sciences in 2009. C57BL/6 and DBA/2 mixed background. |
Strain description | These transgenic mice express an Archaerhodopsin/EGFP fusion protein under the control of the Orexin promoter. |
Colony maintenance | Carrier x Carrier [or Crossing to Jcl:B6D2F1]Tg homozygous and hemizygous mice are viable and fertile. |
References | J. Neuroendocrinol., 22(7): 818-824 (2010). 20456607 Behav. Brain Res., 255:64-74 (2013). 23707248 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter ArchArchaerhodopsinUNArchhuman orexin (hypocretin) promoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter EGFPEnhanced Green Fluorescent Protein (Aequorea victoria)UNEGFP |
Ordering Information | |
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Donor DNA | human orexin (hypocretin) promoter, Halorubrum Archaerhodopsin, jellyfish GFP cDNA, mouse Protamine I polyA |
Research application | Fluorescent Proteins/lacZ System Neurobiology Research |
Specific Term and Conditions | Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. |
Depositor | Akihiro Yamanaka (Nagoya University) |
Strain Status | Frozen sperm |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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Williams RH, Tsunematsu T, Thomas AM, Bogyo K, Yamanaka A, Kilduff TS. Transgenic Archaerhodopsin-3 Expression in Hypocretin/Orexin Neurons Engenders Cellular Dysfunction and Features of Type 2 Narcolepsy. J Neurosci 39(47) 9435-9452(2019) 31628177 |