Strain Information | |
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Image | |
BRC No. | RBRC05847 |
Type | Targeted Mutation and Transgene |
Species | Mus musculus |
Strain name | B6.Cg-Ednrb<tm1Ywa> Tg(DBH-Ednrb)Ywa Tg(Dct-lacZ)A12Jkn |
Former Common name | Ednrb(-/-); DBH-Ednrb; Dct-LacZ+/+, B6-Ednrb KO/DBH-Ednrb/Dct-lacZ |
H-2 Haplotype | |
ES Cell line | JH1 [129S7/SvEvBrd] |
Background strain | |
Appearance | |
Strain development | Developed by Masashi Kato, Nagoya University Graduate School of Medicine. C57BL/6 background. Ddnrb KO and DBH-Ednrb Tg alleles were generated by Masashi Yanagisawa, Howard Hughes Medical Institute. Dct-lacZ Tg allele was generated by Ian Jackson, Western General Hospital. |
Strain description | Ednrb KO/DBH-Ednrb Tg/Dct-lacZ Tg mice. Ednrb homozygous and DBH-Ednrb Tg mice are viable due to a lethal megacolon phenotype of Ednrb KO mice are rescued by the DBH-Ednrb expression. These mice also express lacZ in melanocytes under the control of Dct promoter. |
Colony maintenance | Ednrb KO : Homozygote x Heterozygote ; DBH-Ednrb : Carrier x Carrier ; Dct-lacZ : Carrier x Carrier (Homozygote x Homozygote) |
References | J. Clin. Invest., 102, 1092-1101 (1998). 9739043 Dev. Biol., 192, 99-107 (1997). 9405100 Pigment Cell Res., 19, 644-645 (2006). 17083491 Cell, 79, 1267-1276 (1994). 8001159 |
Health Report | |
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Examination Date / Room / Rack | 2023/04/10Room:3-ARack:I 2023/01/10Room:3-ARack:I 2022/10/11Room:3-ARack:I 2022/07/11Room:3-ARack:I |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)14neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter) Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Ednrbendothelin receptor type B (Rat)UNEdnrbhuman dopamine beta hydroxylase (DBH) promoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter lacZbeta-galactosidase (E. coli)UNmouse dopachrome tautomerase promoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter SV40 splice and polyadenylation signalsUN |
Ordering Information | |
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Donor DNA | Mus msuculus PGK promoter, Escherichia coli Neomycin resistance gene, Rattus norvegicus Endothelin receptor beta, Homo sapiens DBH-promoter, Escherichia coli LacZ, Mus msuculus Dct promoter, SV40 splice and polyadenylation signals |
Research application | Fluorescent Proteins/lacZ System Mouse Models for Human Disease |
Specific Term and Conditions | Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. The DEPOSITOR of BIOLOGICAL RESOURCE must obtain a consent on use of it from the DEVELOPER of the Ednrbtm1Ywa and Tg(DBH-Ednrb)Ywa (Professor Masashi Yanagisawa, University of Tsukuba (yanagisawa.masa.fu@u.tsukuba.ac.jp) and the DEVELOPER of Tg(Dct-lacZ)A12Jkn (Professor Ian J.Jackson, Western General Hospital(Edinburgh, UK)(ian.jackson@igmm.ed.ac.uk) before providing the mice to the RECIPIENT. |
Depositor | Masashi Kato (Chubu University) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved embryos (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |