Strain Data Sheet
ANIMAL SEARCH SYSTEM
Data update: Jul 19, 2019
Health Report   PCR Protocol   Thawing Method
embryos / sperm >
  Mouse Marking
Figure
  Order
RBRC No. RBRC05847  
Type Targeted Mutation and TransgeneCartagena  
Species Mus musculus  
Strain name B6.Cg-Ednrb<tm1Ywa> Tg(DBH-Ednrb)Ywa Tg(Dct-lacZ)A12Jkn  
Former Common name Ednrb(-/-); DBH-Ednrb; Dct-LacZ+/+, B6-Ednrb KO/DBH-Ednrb/Dct-lacZ  
H-2 Haplotype No Data  
ES cell line JH1 [129S7/SvEvBrd]  
Background strain No Data  
Appearance
 1 Appearance No Data  
Genotype No Data  
Strain development Developed by Masashi Kato, Nagoya University Graduate School of Medicine. C57BL/6 background. Ddnrb KO and DBH-Ednrb Tg alleles were generated by Masashi Yanagisawa, Howard Hughes Medical Institute. Dct-lacZ Tg allele was generated by Ian Jackson, Western General Hospital.  
Strain description Ednrb KO/DBH-Ednrb Tg/Dct-lacZ Tg mice. Ednrb homozygous and DBH-Ednrb Tg mice are viable due to a lethal megacolon phenotype of Ednrb KO mice are rescued by the DBH-Ednrb expression. These mice also express lacZ in melanocytes under the control of Dct promoter.  
Colony maintenance Ednrb KO : Homozygote x Heterozygote ; DBH-Ednrb : Carrier x Carrier ; Dct-lacZ : Carrier x Carrier (Homozygote x Homozygote)  
Health Report
Gene Details
Promoter No Data  
 1 Symbol Ednrb  
Symbol name endothelin receptor type B  
Chromosome 14  
Common name No Data  
Symbol description No Data  
 2 Symbol No Data  
Symbol name SV40 splice and polyadenylation signals  
Chromosome UN  
Common name No Data  
Symbol description No Data  
Promoter human dopamine beta hydroxylase (DBH) promoter  
 3 Symbol Ednrb  
Symbol name endothelin receptor type B (Rat)  
Chromosome UN  
Common name No Data  
Symbol description No Data  
Promoter mouse dopachrome tautomerase promoter  
 4 Symbol lacZ  
Symbol name beta-galactosidase (E. coli)  
Chromosome UN  
Common name No Data  
Symbol description No Data  
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)  
 5 Symbol neo  
Symbol name neomycin resistance gene (E. coli)  
Chromosome 14  
Common name neo; neomycin;  
Symbol description No Data  
References Cell. 1994 Dec 30;79(7):1267-76.


  
Targeted and natural (piebald-lethal) mutations of endothelin-B receptor gene produce megacolon associated with spotted coat color in mice.
Targeted and natural (piebald-lethal) mutations of endothelin-B receptor gene produce megacolon associated with spotted coat color in mice.
Targeted and natural (piebald-lethal) mutations of endothelin-B receptor gene produce megacolon associated with spotted coat color in mice.
Targeted and natural (piebald-lethal) mutations of endothelin-B receptor gene produce megacolon associated with spotted coat color in mice.
Research applications Fluorescent Proteins/lacZ System,
Mouse Models for Human Disease  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
The DEPOSITOR of BIOLOGICAL RESOURCE must obtain a consent on use of it from the DEVELOPER of the Ednrbtm1Ywa and Tg(DBH-Ednrb)Ywa (Professor Masashi Yanagisawa, University of Tsukuba (yanagisawa.masa.fu@u.tsukuba.ac.jp) and the DEVELOPER of Tg(Dct-lacZ)A12Jkn (Professor Ian J.Jackson, Western General Hospital(Edinburgh, UK)(ian.jackson@igmm.ed.ac.uk) before providing the mice to the RECIPIENT.  
Additional information
 1 No Data  
 2 Genotyping protocol <PCR>  
Depositor Kato, Masashi (Chubu University College of Life and Health Sciences)  Kato, Masashi 
Strain Status /
Availability
(Expected delivery)

back Back
RIKEN BioResource Research Center

English page click here ANIMAL SEARCH SYSTEM