Strain Data Sheet
ANIMAL SEARCH SYSTEM
Data update: Apr 19, 2019
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RBRC No. RBRC05850  
Type Targeted MutationCartagena  
Species Mus musculus  
Strain name B6.Cg-Flg<tm1Amag>/Rbrc  
Former Common name B6-FLG KO, B6-Flg KO, B6.Cg-Flg<tm1>  
H-2 Haplotype No Data  
ES cell line iTL BA1[(C57BL/6N x 129S6/SvEvTac)F1]  
Background strain C57BL/6JJcl  
Appearance
 1 Appearance No Data  
Genotype No Data  
Strain development Developed by Dr. Akiharu Kubo and Masayuki Amagai, Keio University School of Medicine in 2008. BA1 ES cells were used. The mutant mice were backcrossed to C57BL/6J by using speed congenic method (100 makers were replaced to C57BL/6 type). C57BL/6J background (RBRC05850), BALB/c (RBRC05851).  
Strain description Flg, filaggrin gene knockout mice. The start codon in exon 2 and in-frame ATG sequences of exon 3 were replaced with a PGK-neo cassette. Homozygous mutant mice are viable, but exhibit dry scaly skin.  
Colony maintenance Heterozygote x Homozygote [or Crossing to C57BL/6JJcl]  
Health Report
Gene Details
Promoter No Data  
 1 Symbol Flg  
Symbol name filaggrin  
Chromosome 3  
Common name No Data  
Symbol description No Data  
 2 Symbol Frt  
Symbol name yeast FRT (flippase recombination target) site  
Chromosome 3  
Common name No Data  
Symbol description No Data  
 3 Symbol Frt  
Symbol name yeast FRT (flippase recombination target) site  
Chromosome 3  
Common name No Data  
Symbol description No Data  
 4 Symbol loxP  
Symbol name phage P1 loxP  
Chromosome 3  
Common name No Data  
Symbol description No Data  
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)  
 5 Symbol neo  
Symbol name neomycin resistance gene (E. coli)  
Chromosome 3  
Common name neo; neomycin;  
Symbol description No Data  
References   
Research applications Dermatology Research,
Mouse Models for Human Disease  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT is requested to submit the abstract of the research project to the DEPOSITOR, and shall agree to decide the co-authorship of the DEPOSITOR based agreement with the DEPOSITOR in the case of any publications based on the results from the use of the BIOLOGICAL RESOURCE.  
Additional information
 1 Mouse of the Month Sep 2013  
 2 Genotyping protocol <PCR>  
Depositor Amagai, Masayuki (Keio University)  Amagai, Masayuki 
Strain Status /
Availability
(Expected delivery)

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