Strain Data Sheet
ANIMAL SEARCH SYSTEM
Data update: Aug 20, 2019
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RBRC No. RBRC05893  
Type TransgeneCartagena  
Species Mus musculus  
Strain name B6.Cg-Tg(AKT/Ert2,EGFP)1Nkn  
Former Common name Akt-Mer Tg, B6-AKT/Ert2, GFP Tg  
H-2 Haplotype No Data  
Background strain No Data  
Appearance
 1 Appearance No Data  
Genotype No Data  
Strain development Developed by Toru Nakano, Graduate School of Medicine, Osaka University. The mice were backcrossed to C57BL/6N over 8 times by Shigeo Koyasu, School of Medicine, Keio University.  
Strain description AKT-Mer transgenic mice. These transgenic mice express a fusion protein composed of a myristoylated constitutively active form of human Akt and the ligand-binding domain of a mutant estrogen receptor under the control of the CAG promoter. The Akt-Mer was also fused to IRES-EGFP, can be monitored Akt-Mer expression by GFP fluorescence.  
Colony maintenance Carrier x Noncarrier [C57BL/6NCrSlc]  
Health Report
Gene Details
Promoter No Data  
 1 Symbol EGFP  
Symbol name Enhanced Green Fluorescent Protein, F64L and S65T (Jellyfish)  
Chromosome UN  
Common name No Data  
Symbol description No Data  
 2 Symbol ERT2  
Symbol name estrogen receptor (ER), human ER with the triple mutation G400V/M543A/L544A)  
Chromosome UN  
Common name No Data  
Symbol description No Data  
 3 Symbol HA  
Symbol name human influenza virus HA (hemagglutinin) tag sequence  
Chromosome UN  
Common name No Data  
Symbol description No Data  
 4 Symbol IRES  
Symbol name internal ribosomal entry site (EMCV)  
Chromosome UN  
Common name No Data  
Symbol description No Data  
Promoter CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)  
 5 Symbol AKT  
Symbol name thymoma viral proto-oncogene (human)  
Chromosome UN  
Common name No Data  
Symbol description No Data  
References Oncogene. 2007 Feb 12;.
  
Akt activation induces epidermal hyperplasia and proliferation of epidermal progenitors.
Akt activation induces epidermal hyperplasia and proliferation of epidermal progenitors.
Research applications Cancer Research,
Fluorescent Proteins/lacZ System  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEVELOPER, Professor Toru Nakano of Osaka University.  
Additional information
 1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.
Lab HP  
 2 Genotyping protocol <PCR>  
Depositor Koyasu, Shigeo (Keio University)  Koyasu, Shigeo 
Strain Status /
Availability
(Expected delivery)

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