Strain Data Sheet

RBRC05917

Strain Information

Image
BRC No.RBRC05917
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129S2-Mir200b<tm1.1Osb> Mir429<T30C>
Former Common namemiR-DKO, Mir200b<tm1.1Osb> Mir429<T30COsb>/39
H-2 Haplotype
ES Cell lineD3 [129S2/SvPas]
Background strain
Appearance
Strain developmentDeveloped by Hidetoshi Hasuwa and Masaru Okabe, Research Institute for Microbial Diseases, Osaka University in 2003. The mutant mice were backcrossed to C57BL/6N over 7 times.
Strain descriptionmiR200b knockout mice having a point mutation in miR429. Homozygous mutant females show a severe infertility due to abnormal ovulation.
Colony maintenanceHeterozygote x Wild-type[C57BL/6NJcl]
ReferencesScience, 341, 71-73 (2013). 23765281

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Mir200bmicroRNA 200b4Mir200b<tm1.1Osb>targeted mutation 1.1, Research Institute for Microbial Diseases, Osaka University

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Mir429microRNA 4294Mir429<m1Osb>T30C

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP4loxP

Ordering Information

Donor DNAPhage P1 loxP site, mouse miR-200b-miR-200a-miR-429 genomic DNA
Research applicationCre/loxP system
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Science, 341, 71-73 (2013).RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University (https://www.ccb.osaka-u.ac.jp/en/). The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
DepositorMasahito Ikawa (Osaka University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)
Lab HP
Genotyping protocol -PCR-

BRC mice in Publications

No Data