Strain Information | |
---|---|
Image | |
BRC No. | RBRC05923 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6;129P2-Eed<tm1Homy> |
Former Common name | Eed cKO mice |
H-2 Haplotype | |
ES Cell line | E14 [129P2/OlaHsd] |
Background strain | |
Appearance | |
Strain development | Developed by Hiroaki Honda, Research Institute for Radiation Biology and Medicine, Hiroshima University in 2012. E14 ES cells (129P2/OlaHsd) were used. The mutant mice were crossed to C57BL/6 mice. Neomycin selection cassette was removed by crossing with FLPe mice. |
Strain description | Eed floxed mice. Exon 6 of the Eed gene was flanked by loxP sites. |
Colony maintenance | Heterozygote x Wild-type [C57BL/6NCrlCrlj] |
References | Leukemia, 26, 2557-2560 (2012). 22733077 |
Health Report | |
---|---|
Examination Date / Room / Rack |
Gene | |
---|---|
Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site7Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP7loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP7loxP |
Ordering Information | |
---|---|
Donor DNA | mouse Eed genomic DNA, yeast FRT (flipase recombination target) site, phage P1 loxP |
Research application | Cre/loxP system Developmental Biology Research FLP/frt system Mouse Models for Human Disease |
Specific Term and Conditions | Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. |
Depositor | Hiroaki Honda (Tokyo Women's Medical University) |
Strain Status | Frozen sperm |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
---|
No Data |