Strain Data Sheet
ANIMAL SEARCH SYSTEM
Data update: Jun 20, 2019
Health Report   PCR Protocol   Thawing Method
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RBRC No. RBRC05927  
Type Targeted MutationCartagena  
Species Mus musculus  
Strain name B6.Cg-Map3k5<tm1Hijo> Map3k15<tm1Hijo>  
Former Common name ASK1/3-DKO  
H-2 Haplotype No Data  
Background strain C57BL/6JJcl  
Appearance
 1 Appearance No Data  
Genotype No Data  
Strain development Developed by Hidenori Ichijo, Graduate School of Pharmaceutical Sciences, The University of Tokyo. The double mutant mice were generated by crossing with Map3k5 KO mice and Map3k15 KO mice. C57BL/6J background.  
Strain description Map3k5 (ASK1) and Map3k15 (ASK3) genes double knockout mice. The coding region of Exon 1 of Map3k5 gene was replaced with a GFP/PGK-neo cassette (GFP expression was not observed). A part of exon 1 of Map3k15 was replaced with a neo cassette. Double homozygous mutant mice are viable and fertile.  
Colony maintenance Map3k5: Homozygote x Homozygote; Map3k15: Homozygote (female) x Hemizygote (male) [or Crossing to C57BL/6JJcl]  
Health Report
Gene Details
Promoter No Data  
 1 Symbol Map3k5  
Symbol name mitogen-activated protein kinase kinase kinase 5  
Chromosome 10  
Common name No Data  
Symbol description No Data  
 2 Symbol GFP  
Symbol name Green Fluorescent Protein (Jellyfish)  
Chromosome 10  
Common name No Data  
Symbol description No Data  
 3 Symbol GH  
Symbol name Growth hormone polyA (Bovine)  
Chromosome 10  
Common name No Data  
Symbol description No Data  
 4 Symbol Map3k15  
Symbol name mitogen-activated protein kinase kinase kinase 15  
Chromosome X  
Common name No Data  
Symbol description No Data  
 5 Symbol loxP  
Symbol name phage P1 loxP  
Chromosome X  
Common name No Data  
Symbol description No Data  
 6 Symbol neo  
Symbol name neomycin resistance gene (Klebsiella pneumoniae)  
Chromosome X  
Common name No Data  
Symbol description No Data  
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)  
 7 Symbol neo  
Symbol name neomycin resistance gene (E. coli)  
Chromosome 10  
Common name No Data  
Symbol description No Data  
References
  

Research applications Apoptosis Research,
Cre/loxP system,
Fluorescent Proteins/lacZ System,
Immunology and Inflammation Research  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use with the results from the use of the BIOLOGICAL RESOURCE.  
Additional information
 1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.  
Depositor Ichijo, Hidenori (The University of Tokyo)  Ichijo, Hidenori 
Strain Status /
Availability
(Expected delivery)

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