Strain Data Sheet
ANIMAL SEARCH SYSTEM
Data update: Jul 19, 2019
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RBRC No. RBRC05931  
Type Targeted MutationCartagena  
Species Mus musculus  
Strain name C57BL/6-Gt(ROSA)26Sor<tm1.1(mCherry-MBD)Osb>  
Former Common name MethylRO (Methylation probe in ROSA26 locus)  
H-2 Haplotype No Data  
ES cell line EGR-G101 [C57BL/6-Tg(CAG/Acr-EGFP)C3-N01-FJ002Osb]  
Background strain No Data  
Appearance
 1 Appearance No Data  
Genotype No Data  
Strain development Developed by Jun Ueda and Kazuo Yamagata, Research Institute for Microbial Diseases, Osaka University. EGR-G101 ES cells derived from C57BL/6-Tg(CAG/Acr-EGFP)C3-N01-FJ002Osb were used. Frt flanked neo was removed by transient expression of FLP recombinase in fertilized eggs of conditional MethylRO mice.  
Strain description ROSA26 mCherry-MBD knock-in mice (MethylRO).  
Colony maintenance Homozygote x Homozygote [or Crossing to C57BL/6NJcl]
Homozygous mutant mice show no obvious abnormality.  
Health Report
Gene Details
Promoter No Data  
 1 Symbol Gt(ROSA)26Sor  
Symbol name gene trap ROSA 26, Philippe Soriano  
Chromosome 6  
Common name No Data  
Symbol description No Data  
 2 Symbol Frt  
Symbol name yeast FRT (flippase recombination target) site  
Chromosome No Data  
Common name No Data  
Symbol description No Data  
 3 Symbol MBD1  
Symbol name methyl-CpG binding domain protein 1 (human)  
Chromosome No Data  
Common name No Data  
Symbol description No Data  
 4 Symbol SA  
Symbol name Splice acceptor (adenovirus)  
Chromosome No Data  
Common name No Data  
Symbol description No Data  
 5 Symbol mCherry  
Symbol name Red Fluorescent Protein (Discosoma sp.)  
Chromosome No Data  
Common name No Data  
Symbol description No Data  
References Dev Biol. 2006 Dec 24;.
Dev Biol. 2007 Dec 1;312(1):419-26. Epub 2007 Sep 29.  
Time-lapse and retrospective analysis of DNA methylation in mouse preimplantation embryos by live cell imaging.
Time-lapse and retrospective analysis of DNA methylation in mouse preimplantation embryos by live cell imaging.
Research applications Cancer Research,
Cell Biology Research,
Fluorescent Proteins/lacZ System  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
will be announced.
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.  
Additional information
 1 No Data  
Depositor Yamagata, Kazuo (Osaka university)  Yamagata, Kazuo 
Strain Status /
Availability
(Expected delivery)

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