Strain Data Sheet

RBRC05944

Strain Information

Image
BRC No.RBRC05944
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6.Cg-Tg(Mt1-RET)304Ina Tg(Dct-LacZ)A12Jkn
Former Common name304RET;Dct-LacZ
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Masashi Kato, Nagoya University Graduate School of Medicine. C57BL/6 background. The Dct-lacZ Tg allele was generated by Ian Jackson, Western General Hospital.
Strain descriptionMt1-RET/Dct-lacZ Tg mice. These mice express the lacZ in melanocytes under the control of Dct promoter and the human RET under the control of the mouse MT1 promoter/enhancer. Mt1-RET homozygous mutant mice are embryonic lethal.
Colony maintenanceMt1-RET: Carrier x Noncarrier; Dct-lacZ: Carrier x Carrier (Homozygote x Homozygote)
ReferencesDev. Biol., 192, 99-107(1997). 9405100
Oncogene, 17, 1885-1888 (1998). 9778055

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
RETret proto-oncogene (human)UNRETmouse metallothionein-I promoter-enhancer

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
lacZbeta-galactosidase (E. coli)UNmouse Dct promoter

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 splicing signal-polyadenylation siteUN

Ordering Information

Donor DNAMus musclus Metallothionein-I promoter-enhancer, Homo sapiens RFP/RET cDNA, SV40 splicing signal-polyadenylation site, vector sequence, Mus musuculus Dct promoter, Escherichia coli LacZ
Research applicationCancer Research
Dermatology Research
Fluorescent Proteins/lacZ System
Mouse Models for Human Disease
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorMasashi Kato (Chubu University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data