Strain Data Sheet
ANIMAL SEARCH SYSTEM
Data update: Jun 20, 2019
Health Report   PCR Protocol   Thawing Method
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RBRC No. RBRC05950  
Type Targeted MutationCartagena  
Species Mus musculus  
Strain name B6.Cg-Lrrc32<tm1Hfuj>  
Former Common name GARP-Floxed, B6-Lrrc32 floxed  
H-2 Haplotype No Data  
ES cell line iTL BA1[(C57BL/6N x 129S6/SvEvTac)F1]  
Background strain No Data  
Appearance
 1 Appearance No Data  
Genotype No Data  
Strain development Developed by Hodaka Fujii, Research Institute for Microbial Diseases, Osaka University in 2009. iTL BA1 ES Cells derived from (C57BL/6N x 129S6/SvEvTac)F1 were used. The mutant mice were crossed to C57BL/6.  
Strain description GARP (Lrrc32) gene floxed mice. Exon 1 of the Lrrc32 gene was flanked by loxP sites.  
Colony maintenance Homozygote x Homozygote [or Crossing to C57BL/6JJcl] Homozygous mice are viable and fertile.  
Health Report
Gene Details
Promoter No Data  
 1 Symbol Lrrc32  
Symbol name leucine rich repeat containing 32  
Chromosome 7  
Common name Garp  
Symbol description No Data  
 2 Symbol Frt  
Symbol name yeast FRT (flippase recombination target) site  
Chromosome 7  
Common name No Data  
Symbol description No Data  
 3 Symbol loxP  
Symbol name phage P1 loxP  
Chromosome 7  
Common name No Data  
Symbol description No Data  
References   
Research applications Cre/loxP system,
FLP/frt system  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
J. Immunol., ,190:, 5506-5515, (2013).
RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University (http://www.uic.osaka-u.ac.jp/en/index.html). The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.  
Additional information
 1 No Data  
 2 Genotyping protocol <PCR>  
Depositor Fujii, Hodaka (Osaka university)  Fujii, Hodaka 
Strain Status /
Availability
(Expected delivery)

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