Strain Information | |
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Image | |
BRC No. | RBRC05977 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6.129P2-Mmp14<tm2Ski> |
Former Common name | MT1-MMP(MMP14) knockout mice |
H-2 Haplotype | |
ES Cell line | E14 [129P2/OlaHsd] |
Background strain | |
Appearance | |
Strain development | Developed by Motoharu Seiki and Motoya Katsuki, Institute of Medical Science, University of Tokyo. E14 ES cells were used. The mutant mice were backcrossed to C57BL/6J. |
Strain description | T1-MMP (Mmp14) gene knockout mice (lacZ knock-in mice). Exons 1-5 were replaced with a NLS-lacZ cassette. Homozygous mutant mice die within 2-3 weeks after birth. |
Colony maintenance | Heterozygote x Wild-type [C57BL/6JJcl] |
References | Genes Cells., 14, 617-626 (2009). 19371380 J. Cell Sci., 120, 1607-1614 (2007). 17405818 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Mmp14matrix metallopeptidase 14 (membrane-inserted)14Mmp14<tm2Ski>targeted mutstion 2, Motoharu Seiki Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter gptguanine phosphoribosyltransferase14gptmouse phosphoglycerate kinase promoter (PGK promoter) Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter lacZbeta-galactosidase (E. coli)14 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)14herpes simplex virus thymidine kinase promoter (HSV tk promoter) Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter nlsSV40 large T antigen nuclear localization signal (NLS)14nls |
Ordering Information | |
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Donor DNA | SV40 nuclear localization signal (NLS), E. coli LacZ, mouse Phosphoglcerate kinase promoter (PGK promoter), E. coli gpt, HSV Thymidine kinase promoter, E.coli neomycin resistance gene, SV40 PolyA signal |
Research application | Dermatology Research Developmental Biology Research Fluorescent Proteins/lacZ System |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Genes Cells., 14, 617-626 (2009). |
Depositor | Motoharu Seiki (The University of Tokyo) |
Strain Status | Frozen sperm |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |