Strain Data Sheet
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Data update: Jun 20, 2019
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RBRC No. RBRC05977  
Type Targeted MutationCartagena  
Species Mus musculus  
Strain name B6.129P2-Mmp14<tm2Ski>  
Former Common name MT1-MMP(MMP14) knockout mice  
H-2 Haplotype No Data  
ES cell line E14 [129P2/OlaHsd]  
Background strain No Data  
Appearance
 1 Appearance No Data  
Genotype No Data  
Strain development Developed by Motoharu Seiki and Motoya Katsuki, Institute of Medical Science, University of Tokyo. E14 ES cells were used. The mutant mice were backcrossed to C57BL/6J.  
Strain description T1-MMP (Mmp14) gene knockout mice (lacZ knock-in mice). Exons 1-5 were replaced with a NLS-lacZ cassette. Homozygous mutant mice die within 2-3 weeks after birth.  
Colony maintenance Heterozygote x Wild-type [C57BL/6JJcl]  
Health Report
Gene Details
Promoter No Data  
 1 Symbol Mmp14  
Symbol name matrix metallopeptidase 14 (membrane-inserted)  
Chromosome 14  
Common name No Data  
Symbol description No Data  
 2 Symbol lacZ  
Symbol name beta-galactosidase (E. coli)  
Chromosome 14  
Common name No Data  
Symbol description No Data  
 3 Symbol nls  
Symbol name SV40 large T antigen nuclear localization signal (NLS)  
Chromosome 14  
Common name No Data  
Symbol description No Data  
Promoter herpes simplex virus thymidine kinase promoter (HSV tk promoter)  
 4 Symbol neo  
Symbol name neomycin resistance gene (E. coli)  
Chromosome 14  
Common name No Data  
Symbol description No Data  
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)  
 5 Symbol gpt  
Symbol name guanine phosphoribosyltransferase  
Chromosome 14  
Common name No Data  
Symbol description No Data  
References J Cell Sci. 2007 Apr 12;.
  
Crosstalk between neovessels and mural cells directs the site-specific expression of MT1-MMP to endothelial tip cells.
Crosstalk between neovessels and mural cells directs the site-specific expression of MT1-MMP to endothelial tip cells.
Research applications Dermatology Research,
Developmental Biology Research,
Fluorescent Proteins/lacZ System  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Genes Cells., 14, 617-626 (2009).  
Additional information
 1 No Data  
Depositor Seiki, Motoharu (The Institute of Medical Science, The University of Tokyo)  Seiki, Motoharu 
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