Strain Information | |
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Image | |
BRC No. | RBRC05981 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | ICR;129-Hes5<tm1Fgu> |
Former Common name | Hes5 KO (Neo) ; Hes5 KO |
H-2 Haplotype | |
ES Cell line | R1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>] |
Background strain | |
Appearance | |
Strain development | Developed by Ryoichiro Kageyama, Kyoto University/IGBMC in 1996. R1 ES cells were used. The mutant mice were crossed to ICR. |
Strain description | Hes5 gene knockout mice. 724 bp including the coding region from Met1 to Ala76 (bHLH domain) was replaced with a PGK-neo cassette. |
Colony maintenance | Heterozygote x Wild-type [or Crossing to Slc:ICR] |
References | Development. 2000 127(11):2323-32. 10804175 EMBO J. 1999 18(8):2196-207. 10205173 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)4mouse phosphoglycerate kinase promoter (PGK promoter) |
Ordering Information | |
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Donor DNA | mouse phosphoglycerate kinase promoter (PGK promoter), Neo (E. coli), mouse Hes5 genomic DNA |
Research application | |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. EMBO J. 1999 18(8):2196-207.In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT of BIOLOGICAL resource shall obtain a prior written consent on use of it from the DEPOSITOR Dr. Ryoichiro Kageyama (E-mail: rkageyam@virus.kyoto-u.ac.jp ). (No approval form (Form D) is necessary). |
Depositor | Drs. Itaru Imayoshi and Ryoichiro Kageyama (Kyoto University) |
Strain Status | Frozen embryos |
Strain Availability | Recovery and QC required prior to distribution |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |