Strain Data Sheet
ANIMAL SEARCH SYSTEM
Data update: Jun 20, 2019
Health Report   PCR Protocol   Thawing Method
embryos / sperm >
  Mouse Marking
Figure
  Order
RBRC No. RBRC05982  
Type Targeted Mutation CongenicCartagena  
Species Mus musculus  
Strain name ICR.Cg-Hes5<tm1Kag>  
Former Common name Hes5 KO (LacZ LacZ knock-in) ; Hes5 KO/lacZ  
H-2 Haplotype No Data  
ES cell line TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]  
Background strain No Data  
Appearance
 1 Appearance No Data  
Genotype No Data  
Strain development Developed by Ryoichiro Kageyama, Institute for Virus Research, Kyoto University in 2002. TT2 ES cells were used. The mutant mice were crossed to ICR.  
Strain description Hes5 gene nls-lacZ targeted reporter mice. The entire coding region was replaced with a IRES-nls-lacZ expression cassette.  
Colony maintenance Heterozygote x Wild-type [or Crossing to Slc:ICR]  
Health Report No Data  
Gene Details
Promoter No Data  
 1 Symbol Hes5  
Symbol name hairy and enhancer of split 5 (Drosophila)  
Chromosome 4  
Common name No Data  
Symbol description No Data  
 2 Symbol Ires  
Symbol name internal ribosomal entry site (EMCV)  
Chromosome 4  
Common name No Data  
Symbol description No Data  
 3 Symbol lacZ  
Symbol name beta-galactosidase (E. coli)  
Chromosome 4  
Common name No Data  
Symbol description No Data  
 4 Symbol nls  
Symbol name SV40 large T antigen nuclear localization signal (NLS)  
Chromosome 4  
Common name No Data  
Symbol description No Data  
 5 Symbol poly A  
Symbol name polyadenylic acid additional signal  
Chromosome 4  
Common name No Data  
Symbol description No Data  
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)  
 6 Symbol Puro  
Symbol name puromycin resistance gene (Streptomyces alboniger)  
Chromosome 4  
Common name No Data  
Symbol description No Data  
References   
Research applications No Data  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
J Neurosci. 2010 30(9):3489-98.
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT of BIOLOGICAL resource shall obtain a prior written consent on use of it from the DEPOSITOR Dr. Ryoichiro Kageyama (E-mail: rkageyam@virus.kyoto-u.ac.jp ). (No approval form (Form D) is necessary).  
Additional information
 1 No Data  
Depositor Kageyama, Ryoichiro (Institute for Virus Research, Kyoto University)  Kageyama, Ryoichiro 
Strain Status /
Availability
(Expected delivery)

back Back
RIKEN BioResource Research Center

English page click here ANIMAL SEARCH SYSTEM