Strain Data Sheet
RBRC05990
Strain Information | |
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| Image | |
| BRC No. | RBRC05990 |
| Type | Transgene |
| Species | Mus musculus |
| Strain name | ICR;D2-Tg(Hes7-Hes7,venus)1Kag |
| Former Common name | Hes7 Tg ; Hes7-Hes7/venus Tg |
| H-2 Haplotype | |
| ES Cell line | |
| Background strain | |
| Appearance | |
| Strain development | Developed by Ryoichiro Kageyama, Institute for Virus Research, Kyoto University in 2003. The transgene was injected into the pronuclei of DBA/2 fertilized eggs. |
| Strain description | Hes7 transgenic mice Transgene composed of 5.4kb Hes7 promoter-1st exon-1st intron-2nd exon-Hes7 cDNA(ORF of the 1st&2nd exons are deleted)-IRES-Venus-SV40 polyA Hes7 expression is up-regulated and initially oscillates but later becomes sustained. |
| Colony maintenance | Carrier x Noncarrier [or Crossing to Slc:ICR] |
| References | Dev. Cell., 13(2):298-304 (2007). 17681139 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |
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| Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Hes7hairy and enhancer of split 7 (Drosophila)UNHes7 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Iresinternal ribosomal entry site (EMCV)UN Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter VenusYellow Fluorescent Protein (Aequorea victoria)UNVenus Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter SV40 polyAUN |
Ordering Information | |
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| Donor DNA | mouse Hes7 promoter, 1st exon, 1st intron and 2nd exon of Hes7 gene, EMCV (Encephalomyocarditis virus) internal ribosomal entry site (IRES), Jellyfish Venus, SV40 polyA |
| Research application | |
| Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Dev. Cell., 13(2):298-304 (2007).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT of BIOLOGICAL resource shall obtain a prior written consent on use of it from the DEPOSITOR Dr. Ryoichiro Kageyama (E-mail: rkageyam@virus.kyoto-u.ac.jp ). (No approval form (Form D) is necessary). In publishing the research results obtained by use of the BIOLOGICAL RESOURCE containing Venus, an acknowledgment to Dr. Atsushi Miyawaki and a citation of the following literature(s) designated by the DEPOSITOR are requested. Nat. Biotechnol., 20, 87-90 (2002). |
| Depositor | Drs. Itaru Imayoshi and Ryoichiro Kageyama (Kyoto University) |
| Strain Status |  Frozen embryos |
| Strain Availability |  Recovery and QC required prior to distribution |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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| No Data |