Strain Data Sheet
Data update: Jun 20, 2019
Health Report   PCR Protocol   Thawing Method
embryos / sperm >
  Mouse Marking
RBRC No. RBRC05999  
Type TransgeneCartagena  
Species Mus musculus  
Strain name C57BL/6-Tg(Nes-cre/ERT2)5-1Imayo  
Former Common name Nestin-CreERT2 Line5-1 ; B6-Nes-CreER#5-1  
H-2 Haplotype No Data  
Background strain C57BL/6JJcl  
 1 Appearance No Data  
Genotype No Data  
Strain development Developed by Itaru Imayoshi and Ryoichiro Kageyama, Institute for Virus Research, Kyoto University in 2005. The transgene was injected into the pronuclei of C57BL/6 fertilized eggs.  
Strain description Nestin-CreER transgenic mice. CreERT2 is expressed by neural stem/progenitor cells. CreERT2-expression level is low.  
Colony maintenance Carrier x Noncarrier [C57BL/6JJcl]  
Health Report
Gene Details
Promoter No Data  
 1 Symbol ERT2  
Symbol name estrogen receptor (ER), human ER with the triple mutation G400V/M543A/L544A)  
Chromosome UN  
Common name No Data  
Symbol description No Data  
 2 Symbol PolyA  
Symbol name SV40 PolyA signal  
Chromosome UN  
Common name No Data  
Symbol description No Data  
Promoter rat Nestin promoter, Nestin 2nd intron enhancer  
 3 Symbol cre  
Symbol name Phage P1 Cre recombinase  
Chromosome UN  
Common name No Data  
Symbol description No Data  
References Genesis. 2006 May;44(5):233-8.  
Temporal regulation of Cre recombinase activity in neural stem cells.
Research applications Cre/loxP system  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Genesis. 2006 44(5):233-8.
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT of BIOLOGICAL resource shall obtain a prior written consent on use of it from the DEPOSITOR Dr. Itaru Imayoshi (E-mail: and Ryoichiro Kageyama (E-mail: ). (No approval form (Form D) is necessary).  
Additional information
 1 No Data  
 2 Genotyping protocol <PCR>  
Depositor Imayoshi, Itaru (Institute for Virus Research, Kyoto University)  Imayoshi, Itaru 
Strain Status /
(Expected delivery)

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