Strain Information | |
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Image | |
BRC No. | RBRC06008 |
Type | Targeted Mutation Congenic |
Species | Mus musculus |
Strain name | ICR.Cg-Hes5<tm1(venus)Imayo> |
Former Common name | Venus-Hes5 fusion Knock-in ; Hes5/venus KI |
H-2 Haplotype | |
ES Cell line | TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1] |
Background strain | |
Appearance | |
Strain development | Developed by Itaru Imayoshi and Ryoichiro Kageyama, Institute for Virus Research, Kyoto University in 2010. TT2 ES cells were used. The mutant mice were crossed to ICR. |
Strain description | Venus/Hes5 knock-in mice. Venus-Hes5 fusion protein is expressed from Hes5 locus. |
Colony maintenance | Heterozygote x Wild-type [or Crossing to Slc:ICR] |
References | Science, 342(6163):1203-8. doi: 10.1126/science.1242366. 24179156 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Hes5hairy and enhancer of split 5 (Drosophila)4Hes5<tm1(venus)Imayo>targeted mutation 1, Itaru Imayoshi Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter FLAGFLAG tag (synthetic)4FLAG Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site4Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter VenusYellow Fluorescent Protein (Aequorea victoria)4Venus |
Ordering Information | |
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Donor DNA | FLAG tag (synthetic), Jellyfish venus cDNA, Yeast Frt (flippase recombination target) site, mouse Hes5 genomic DNA |
Research application | FLP/frt system Fluorescent Proteins/lacZ System |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Science, 342(6163):1203-8. doi: 10.1126/science.1242366.In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT of BIOLOGICAL resource shall obtain a prior written consent on use of it from the DEPOSITOR Dr. Itaru Imayoshi (E-mail: iimayosh@virus.kyoto-u.ac.jp) and Ryoichiro Kageyama (E-mail: rkageyam@virus.kyoto-u.ac.jp ). (No approval form (Form D) is necessary). In publishing the research results obtained by use of the BIOLOGICAL RESOURCE containing Venus, an acknowledgment to Dr. Atsushi Miyawaki and a citation of the following literature(s) designated by the DEPOSITOR are requested. Nat. Biotechnol., 20, 87-90 (2002). |
Depositor | Drs. Ryoichiro Kageyama and Itaru Imayoshi (Kyoto University) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |