Strain Data Sheet

RBRC06031

Strain Information

Image
BRC No.RBRC06031
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129S2-Kpna1<tm1.2Yyo>
Former Common nameImpa5-
H-2 Haplotype
ES Cell lineD3 [129S2/SvPas]
Background strain
Appearance
Strain developmentDeveloped by Tetsuji Moriyama and Yoshihiro Yoneda, Graduate School of Frontier Biosciences, Osaka University in 2006. A neo cassette was removed by crossing with CAG-FLP mice (B6 background), and then exons 2 and 3 were removed by crossing with CAG-Cre mice (B6 background). The mutant mice were backcrossed to C57BL/6 over 10 times.
Strain descriptionKpna1 (IPOA5, Impoirtin alpha 5) gene floxed mice. Exons 2 and 3 were flanked by loxP sites.
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJcl]
ReferencesFEBS J., 278, 1561-1572 (2011). 21371262

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Kpna1karyopherin (importin) alpha 116Kpna1<tm1.2Yyo>targeted mutation 1, Yoshihiro Yoneda

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP16loxP

Ordering Information

Donor DNAphage P1 loxP site, Mouse Kpna1 genomic DNA
Research applicationCell Biology Research
Cre/loxP system
Immunology and Inflammation Research
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. FEBS J., 278, 1561-1572 (2011).In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must contact the DEPOSITOR and notify him the research content. If the DEPOSITOR judges that the research should be a collaborative work, then the RECIPIENT must include the DEPOSITOR/DEVELOPER as co-authors.
DepositorMasahiro Oka (National Institutes of Biomedical Innovation, Health and Nutrition)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data