Strain Information | |
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Image | |
BRC No. | RBRC06031 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6.129S2-Kpna1<tm1.2Yyo> |
Former Common name | Impa5- |
H-2 Haplotype | |
ES Cell line | D3 [129S2/SvPas] |
Background strain | |
Appearance | |
Strain development | Developed by Tetsuji Moriyama and Yoshihiro Yoneda, Graduate School of Frontier Biosciences, Osaka University in 2006. A neo cassette was removed by crossing with CAG-FLP mice (B6 background), and then exons 2 and 3 were removed by crossing with CAG-Cre mice (B6 background). The mutant mice were backcrossed to C57BL/6 over 10 times. |
Strain description | Kpna1 (IPOA5, Impoirtin alpha 5) gene floxed mice. Exons 2 and 3 were flanked by loxP sites. |
Colony maintenance | Heterozygote x Wild-type [C57BL/6JJcl] |
References | FEBS J., 278, 1561-1572 (2011). 21371262 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP16loxP |
Ordering Information | |
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Donor DNA | phage P1 loxP site, Mouse Kpna1 genomic DNA |
Research application | Cell Biology Research Cre/loxP system Immunology and Inflammation Research |
Specific Term and Conditions | Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. FEBS J., 278, 1561-1572 (2011).In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must contact the DEPOSITOR and notify him the research content. If the DEPOSITOR judges that the research should be a collaborative work, then the RECIPIENT must include the DEPOSITOR/DEVELOPER as co-authors. |
Depositor | Masahiro Oka (National Institutes of Biomedical Innovation, Health and Nutrition) |
Strain Status | Frozen sperm |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |