Strain Data Sheet
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Data update: Jun 20, 2019
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RBRC No. RBRC06041  
Type Targeted Mutation CongenicCartagena  
Species Mus musculus  
Strain name ICR.Cg-Hcn4<tm1(tTA)>/2Kyo  
Former Common name ICR-Tet-HCN4#1, ICR-Hcn4/tTA KI#2  
H-2 Haplotype No Data  
ES cell line TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]  
Background strain No Data  
Appearance
 1 Appearance No Data  
Genotype No Data  
Strain development Developed by Takahiro Ishii, Noriyuki Nakashima and Harunori Ohmori, Faculty of Medicine, Kyoto University. TT2 ES cells were used. The mutant mice were backcrossed to C57BL/6 and ICR over 20 times, respectively. C57BL/6 background (line 1: RBRC06038, line 2: RBRC06039), ICR background (line 1: RBRC06040, line 2: RBRC06040).  
Strain description Hcn4 tTA knock-in mice.  
Colony maintenance Heterozygote x Wild-type [or Crossing to Slc:ICR] Homozygous mutant mice may be embryonic lethal.  
Health Report No Data  
Gene Details
Promoter No Data  
 1 Symbol tTA  
Symbol name tTA (E. coli tet repressor (TetR), herpes simplex virus (HSV) VP16 activation domain)  
Chromosome 9  
Common name E. coli tet repressor (TetR), herpes simplex virus (HSV)VP16 activation domain  
Symbol description No Data  
 2 Symbol No Data  
Symbol name SV40 PolyA signal  
Chromosome 9  
Common name No Data  
Symbol description No Data  
 3 Symbol GH  
Symbol name human growth hormone gene  
Chromosome 9  
Common name No Data  
Symbol description No Data  
 4 Symbol URA3  
Symbol name Orotidine-5'-phosphate (OMP) decarboxylase (yeast)  
Chromosome 9  
Common name No Data  
Symbol description No Data  
 5 Symbol loxP  
Symbol name phage P1 loxP  
Chromosome 9  
Common name No Data  
Symbol description No Data  
Promoter E. coli tet operator, cytomegalovirus CMV promoter  
 6 Symbol Hcn4  
Symbol name hyperpolarization-activated, cyclic nucleotide-gated K+ 4  
Chromosome 9  
Common name No Data  
Symbol description No Data  
Promoter herpes simplex virus thymidine kinase promoter (HSV tk promoter)  
 7 Symbol neo  
Symbol name neomycin resistance gene (E. coli)  
Chromosome 9  
Common name No Data  
Symbol description No Data  
References   
Research applications Cre/loxP system,
Tet system  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
J. Physiol., 591, 1749-1769 (2013).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
The RECIPIENT agrees to use the BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR/DEVELOPER(S).  
Additional information
 1 No Data  
Depositor Ohmori, Harunori (Graduate School of Medicine, Kyoto University)  Ohmori, Harunori 
Strain Status /
Availability
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