Strain Data Sheet
ANIMAL SEARCH SYSTEM
Data update: Jun 20, 2019
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RBRC No. RBRC06047  
Type Targeted Mutation CongenicCartagena  
Species Mus musculus  
Strain name B6.Cg-Hes1<tm1Imayo>  
Former Common name B6-Hes1-floxed ; B6-Hes1 floxed  
H-2 Haplotype No Data  
ES cell line TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]  
Background strain No Data  
Appearance
 1 Appearance No Data  
Genotype No Data  
Strain development Developed by Itaru Imayoshi and Ryoichiro Kageyama, Institute for Virus Research, Kyoto University in 2007. TT2 ES cells were used. The mutant mice were crossed to C57BL/6 for 6 times.  
Strain description Hes1 gene floxed mice. LoxP sequences were knocked into the first intron and downstream of 3'UTR of Hes1 gene for conditional knock-out study. PGK-Neo sequence was removed.  
Colony maintenance Homozygote x Homozygote [or Crossing to C57BL/6JJcl]  
Health Report
Gene Details
Promoter No Data  
 1 Symbol Hes1  
Symbol name hairy and enhancer of split 1 (Drosophila)  
Chromosome 16  
Common name No Data  
Symbol description No Data  
 2 Symbol loxP  
Symbol name phage P1 loxP  
Chromosome 16  
Common name No Data  
Symbol description No Data  
References Development. 2008 Aug;135(15):2531-41. Epub 2008 Jun 25.  
Hes genes and neurogenin regulate non-neural versus neural fate specification in the dorsal telencephalic midline.
Research applications Cre/loxP system,
Developmental Biology Research  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Development. 2008 135(15):2531-41.
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT of BIOLOGICAL resource shall obtain a prior written consent on use of it from the DEPOSITOR Dr. Itaru Imayoshi (E-mail: iimayosh@virus.kyoto-u.ac.jp) and Ryoichiro Kageyama (E-mail: rkageyam@virus.kyoto-u.ac.jp ). (No approval form (Form D) is necessary).  
Additional information
 1 No Data  
 2 Genotyping protocol <PCR>  
Depositor Imayoshi, Itaru (Institute for Virus Research, Kyoto University)  Imayoshi, Itaru 
Strain Status /
Availability
(Expected delivery)

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