Strain Data Sheet
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Data update: Aug 20, 2019
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RBRC No. RBRC06054  
Type Targeted Mutation and TransgeneCartagena  
Species Mus musculus  
Strain name C57BL/6-Polk<tm3(mpolk)Csk> Tg(gptdelta)1Nmi  
Former Common name inactivated Polk KI and gpt delta double transgenic mice  
H-2 Haplotype No Data  
ES cell line Not specified (ES cell)  
Background strain No Data  
Appearance
 1 Appearance No Data  
Genotype No Data  
Strain development Developed by Takehiko Nohmi, National Institute of Health Sciences. Inactivate Polk KI mice were generated by using C57BL/6N derived ES cells. The mutant mice were crossed with the gpt delta Tg mice (C57BL/6J background) (RBRC00158). C57BL/6J and C57BL/6N mixed background.  
Strain description Inactivate Polk knock-in mice, in which Asp at position 197 and Glu at position 198 of exon 6 were replace with Ala, respectively. This mutant mice also have a gpt delta transgene that serves as a tool for assessing the mutagenicity of environmental chemicals.  
Colony maintenance KI: Heterozygote x Wild-type; Tg: Carrier x Carrier  
Health Report
Gene Details
Promoter No Data  
 1 Symbol Polk  
Symbol name polymerase (DNA directed), kappa  
Chromosome 13  
Common name No Data  
Symbol description No Data  
 2 Symbol Polk  
Symbol name polymerase (DNA directed), kappa  
Chromosome 13  
Common name No Data  
Symbol description No Data  
 3 Symbol cat  
Symbol name E. coli Chloramphenicol acetyltransferase (CAT)  
Chromosome 17  
Common name No Data  
Symbol description No Data  
 4 Symbol gpt Delta  
Symbol name guanine phosphoribosyltransferase  
Chromosome 17  
Common name No Data  
Symbol description No Data  
 5 Symbol loxP  
Symbol name phage P1 loxP  
Chromosome 17  
Common name No Data  
Symbol description No Data  
 6 Symbol red/gam  
Symbol name lambda phage red/gam  
Chromosome 17  
Common name No Data  
Symbol description No Data  
References Unpublished  
Research applications Cancer Research,
Cre/loxP system  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must reach agreement on terms and conditions of use of the mice with the DEPOSITOR and its co-developer, Chugai Pharmaceutical Co., Ltd. (Contact person: Dr. Akira Takeiri, e-mail, takeiriakr@chugai-pharm.co.jp) and must obtain a prior written consent from them and, if necessary, shall enter into an agreement with them.  
Additional information
 1 No Data  
 2 Genotyping protocol <PCR>  
Depositor Noumi, Takehiko (National Institute of Health Sciences)  Noumi, Takehiko 
Strain Status /
Availability
(Expected delivery)

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