Strain Information | |
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Image | |
BRC No. | RBRC06064 |
Type | Gene Trap Congenic |
Species | Mus musculus |
Strain name | B6.Cg-Pknox1<Gt(Ayu21-B132*SACre)2Card> |
Former Common name | Pknox1-SACre,402, B6-Pknox1/Cre Gt#2 |
H-2 Haplotype | |
ES Cell line | TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1] |
Background strain | |
Appearance | |
Strain development | Developed by Masatake Araki, Institute of Molecular Embryology and Genetics, Kumamoto University. KTPU8 ES cells were used. C57BL/6 and CBA mixed. |
Strain description | Pknox1-cre driver mice. The beta-geo gene in the exchangeable gene trap clone of Pknox1 gene was replaced with a cre recombinase gene. |
Colony maintenance | Heterozygote x Wild-type [C57BL/6NJcl] |
References |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Pknox1Pbx/knotted 1 homeobox17Pknox1<Gt(Ayu21-B132*SACre)2Card>gene trap 2, Center for Animal Resources & Development Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter En2En2 SA (mouse En2 intron 2/exon 3 splice acceptor sequence)17En2 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site17Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter crePhage P1 Cre recombinase17cre Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP17loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP17loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter nlsSV40 large T antigen nuclear localization signal (NLS)17nls Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter polyAmouse PGK17polyA Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter puropuromycin acyltransferase (Streptomyces alboniger)17puro |
Ordering Information | |
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Donor DNA | mouse En2 splice acceptor, mouse PGK promoter, mouse PGK polyA signal, Streptomyces alboniger puromycin resistance gene, phage P1 cre recombinase, SV40 nuclear localization signal, yeast FRT (flipase recombination target) site, phage P1 loxP and mutant lox sequence, E. coli pSP73 vector sequence, mouse Pknox1 genomic DNA |
Research application | Cre/loxP system |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. will be announced.In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. |
Depositor | Masatake Araki (Kumamoto University) |
Strain Status | Frozen sperm |
Strain Availability | Recovery and QC required prior to distribution |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |