Strain Data Sheet

RBRC06071

Strain Information

Image
BRC No.RBRC06071
TypeGene Trap CongenicCartagena
SpeciesMus musculus
Strain nameB6.Cg-Mettl2<Gt(Ayu21-W15*SACre)2Card>
Former Common nameMettl2-SACre,135, B6-Mettl2/Cre Gt#2
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain developmentDeveloped by Masatake Araki, Institute of Molecular Embryology and Genetics, Kumamoto University. KTPU8 ES cells were used. C57BL/6 and CBA mixed.
Strain descriptionMettl2-cre driver mice. The beta-geo gene in the exchangeable gene trap clone of Mettl2 gene was replaced with a cre recombinase gene.
Colony maintenanceHeterozygote x Wild-type [C57BL/6NJcl]
References

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Mettl2methyltransferase like 211Mettl2<Gt(Ayu21-W15*SACre)2Card>gene trap 2, Center for Animal Resources & Development

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
En2En2 SA (mouse En2 intron 2/exon 3 splice acceptor sequence)11En2

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site11Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
crePhage P1 Cre recombinase11cre

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP11loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP11loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
nlsSV40 large T antigen nuclear localization signal (NLS)11nls

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
polyAmouse PGK polyA11polyA

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
puropuromycin acyltransferase (Streptomyces alboniger)11puro

Ordering Information

Donor DNAmouse En2 splice acceptor, mouse PGK promoter, mouse PGK polyA signal, Streptomyces alboniger puromycin resistance gene, phage P1 cre recombinase, SV40 nuclear localization signal, yeast FRT (flipase recombination target) site, phage P1 loxP and mutant lox sequence, E. coli pSP73 vector sequence, mouse Mettl2 genomic DNA
Research applicationCre/loxP system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. will be announced.In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorMasatake Araki (Kumamoto University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

BRC mice in Publications

No Data