Strain Data Sheet

RBRC06162

Strain Information

Image
BRC No.RBRC06162
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129-Ednra<tm1Ywa>
Former Common nameETA/129, B6-Ednra KO
H-2 Haplotype
ES Cell lineJH1 [129S7/SvEvBrd]
Background strain
Appearance
Strain developmentDeveloped by Masashi Yanagisawa, The University of Texas Southwestern Medical Center. JH1 ES cells were used to generate the mutant mice. 129 and C57BL/6 mixed background.
Strain descriptionETA (Ednra / endothelin receptor type A) gene knockout mice. Exons 5 and 6 were replaced with a neomycin resistant gene. Homozygous mutant mice are perinatal lethal.
Colony maintenanceHeterozygote x Wild-type [C57BL/6J(Crlj)]
ReferencesDevelopment, 125, 813-824 (1998). 9449664

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Ednraendothelin receptor type A8Ednra<tm1Ywa>targeted mutation 1, Masashi Yanagisawa

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
GHGrowth hormone polyA (Bovine)8GH

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)8mouse RNA polymerase II promoter

Ordering Information

Donor DNAmouse RNA polymerase II promoter, E. coli Neomycin resistance gene, bovine growth hormone polyadenylation site, mouse Ednra genomic DNA
Research applicationDevelopmental Biology Research
Hematological Research
Neurobiology Research
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Development, 125, 813-824 (1998).Depositor limits the use of the BIOLOGICAL RESOURCE to not-for-profit institutions. Depositor limits the use of the BIOLOGICAL RESOURCE to academic purposes. For-profit institutions and recipients who intend to use BIOLOGICAL RESOURCE for commercial research purposes shall acquire prior written consent from the Depositor. Recipient shall acquire prior written consent from the Depositor prior to filing an application for patent, or intellectual property or other rights based on the results of research using the BIOLOGICAL RESOURCE.
DepositorMasashi Yanagisawa (University of Texas Southwestern Medical Center at Dallas)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data