Strain Data Sheet
ANIMAL SEARCH SYSTEM
Data update: Jun 20, 2019
Health Report   PCR Protocol   Thawing Method
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  Mouse Marking
Figure
  Order
RBRC No. RBRC06197  
Type Targeted MutationCartagena  
Species Mus musculus  
Strain name B6.129S4-Cckar<tm1Kym>  
Former Common name CCKAR KO, B6-Cckar KO  
H-2 Haplotype No Data  
ES cell line J1 [129S4/SvJae]  
Background strain No Data  
Appearance
 1 Appearance No Data  
Genotype No Data  
Strain development Developed by Soichi Takiguchi and Akihiro Funakoshi, National Kyusu Cancer Center, Tetsuo Noda, The Cancer Institute, Japanese Foundation for Cancer Research and Kyoko Miyasaka, Tokyo Metropolitan Institute of Gerontology. J1 ES cells were used. The mutant mice were backcrossed to C57BL/6J.  
Strain description Cckar gene knockout mice. Part of exon 1 and all of exon 2 were replaced with a NLS-lacZ neo cassette. Homozygous mutant mice are viable and fertile.  
Colony maintenance Homozygote x Homozygote [or Crossing to C57BL/6JJmsSlc]  
Health Report
Gene Details
Promoter No Data  
 1 Symbol Cckar  
Symbol name cholecystokinin A receptor  
Chromosome 5  
Common name No Data  
Symbol description No Data  
 2 Symbol lacZ  
Symbol name beta-galactosidase (E. coli)  
Chromosome 5  
Common name No Data  
Symbol description No Data  
 3 Symbol nls  
Symbol name SV40 large T antigen nuclear localization signal (NLS)  
Chromosome 5  
Common name No Data  
Symbol description No Data  
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)  
 4 Symbol neo  
Symbol name neomycin resistance gene (E. coli)  
Chromosome 5  
Common name No Data  
Symbol description No Data  
References   
Research applications Fluorescent Proteins/lacZ System,
Neurobiology Research  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Pancres, 24, 276-283 (2002).  
Additional information
 1 No Data  
 2 Genotyping protocol <PCR>  
Depositor Miyasaka, Kyoko (Tokyo Kasei University)  Miyasaka, Kyoko 
Strain Status /
Availability
(Expected delivery)

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