Strain Information | |
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Image | |
BRC No. | RBRC06199 |
Type | Transgene |
Species | Mus musculus |
Strain name | B6.Cg-Tg(NDE-hsp-GCaMP2,lacZ)1Hmd |
Former Common name | NDE-GCaMP2-IZ(1), B6-NDE-GCaMP2/lacZ Tg#1 |
H-2 Haplotype | |
ES Cell line | |
Background strain | |
Appearance | |
Strain development | Developed by Satoko Yoshiba and Hiroshi Hamada, Graduate School of Frontier Biosciences, Osaka University in 2010. The NDE-hsp- GCaMP2,lacZ transgene was injected into the pronuclei of B6C3F1 fertilized eggs. C57BL/6, 129 and C3H mixed background. |
Strain description | This transgenic mouse expresses GCaMP2 and lacZ genes on both sides of the node under the control of the mouse node-specific enhancer. |
Colony maintenance | Carrier x Noncarrier [C57BL/6JJcl] |
References | Science, 338, 226-231 (2012). 22983710 Nat. Biotechnol., 19, 137-141 (2001). 11175727 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter GCaMP2RSET tag, chicken myosin light chain kinase (M13) cDNA, Jellyfish circularly permutated EGFP (cpEGFP) cDNA, rat calmodulin (CaM) cDNAUNGCaMP2mouse node-notochord enhancer, mouse Hsp68 promoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Iresinternal ribosomal entry site (EMCV)UN Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter lacZbeta-galactosidase (E. coli)UN Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter SV40 polyA signalUN |
Ordering Information | |
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Donor DNA | mouse node-notochord enhancer, mouse Hsp68 promoter, GCaMP2(RSET tag, chicken myosin light chain kinase (M13), Jellyfish circularly permutated EGFP (cpEGFP) cDNA, rat calmodulin (CaM) cDNA), Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), E.coli LacZ, SV40 polyA signal |
Research application | Developmental Biology Research Fluorescent Proteins/lacZ System |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Science, 338, 226-231 (2012). |
Depositor | Hiroshi Hamada (RIKEN CDB) |
Strain Status | Frozen embryos |
Strain Availability | Recovery and QC required prior to distribution |
Additional Info. | Necessary documents for ordering: |
BRC mice in Publications |
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No Data |