Strain Data Sheet
ANIMAL SEARCH SYSTEM
Data update: Jul 19, 2019
Health Report   PCR Protocol   Thawing Method
embryos / sperm >
  Mouse Marking
Figure
  Order
RBRC No. RBRC06208  
Type TransgeneCartagena  
Species Mus musculus  
Strain name B6C3-Tg(Cerl2-sGFP*d3*dInt)25Hmd  
Former Common name Cerl2(Cerl2-sGFP-Delta 3'-Delta int)BAC(25)  
H-2 Haplotype No Data  
Background strain No Data  
Appearance
 1 Appearance No Data  
Genotype No Data  
Strain development Developed by Tetsuya Nakamura and Hiroshi Hamada, Graduate School of Frontier Biosciences, Osaka University in 2008. The transgene was injected into the pronuclei of B6C3F1 fertilized eggs. C57BL/6 and C3H mixed background.  
Strain description BAC Cerl2-sGFP (delta 3',delta intron) transgenic mice. The mice were generated by using a BAC clone containing the mouse Cerl2 gene, PR23-421H21. The sGFP (a 300-bp sequence coding for a portion of EGFP) gene was inserted into the exon 2 of in the Cerl2 BAC clone. The 3'-UTR of Cerl2 was replaced by the polyA sequence (250 bp) of SV40, and intron 1of Cerl2 had been excised.  
Colony maintenance Carrier x Noncarrier [or Crossing to Jcl:B6C3F1]  
Health Report No Data  
Gene Details
Promoter No Data  
 1 Symbol polyA  
Symbol name SV40 virus polyA signal sequence  
Chromosome UN  
Common name No Data  
Symbol description No Data  
Promoter mouse Dand5 promoter  
 2 Symbol EGFP  
Symbol name Enhanced Green Fluorescent Protein, F64L and S65T (Jellyfish)  
Chromosome UN  
Common name No Data  
Symbol description No Data  
References   
Research applications Developmental Biology Research,
Fluorescent Proteins/lacZ System  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER.  
Additional information
 1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.  
Depositor Hamada, Hiroshi (RIKEN Center for Developmental Biology)  Hamada, Hiroshi 
Strain Status /
Availability
(Expected delivery)

back Back
RIKEN BioResource Research Center

English page click here ANIMAL SEARCH SYSTEM