Strain Information | |
---|---|
Image | |
BRC No. | RBRC06210 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6.Cg-Pgap3<tm1> |
Former Common name | Pgap3 floxed, B6-Pgap3 floxed |
H-2 Haplotype | |
ES Cell line | EGR-G01 [129S2 x C57BL/6NCr-Tg(CAG/Acr-EGFP)] |
Background strain | |
Appearance | |
Strain development | Developed by Yetao Wang and Taroh Kinoshita, Research Institute for Microbial Diseases, Osaka University in 2007. EGRG01 ES cells derived from (129S2 x C57BL/6CrSlc)F1-Tg(GFP) were used for the generation of floxed mice. The mutant mice were backcrossed to C57BL/6J over 10 times. |
Strain description | Pgap3 gene floxed mice. Exon 7 and 8 were flanked by loxP sites. A FRT flanked neo cassette was inserted after the exon 8. |
Colony maintenance | Heterozygote x Wild-type [C57BL/6JJcl] |
References | Biochem. Biophys. Res. Commun., 417, 1235-1241(2012). 22227195 J. Biol. Chem., 88, 25490-25499 (2013). 23864655 |
Health Report | |
---|---|
Examination Date / Room / Rack | 2024/01/09Room:3-BRack:E 2023/10/10Room:3-BRack:E |
Gene | |
---|---|
Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site11Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site11Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP11loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP11loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)11mouse phosphoglycerate kinase promoter (PGK promoter) |
Ordering Information | |
---|---|
Donor DNA | Phage P1 loxP sites, yeast flippase target sites, mouse phosphoglycerate kinase promoter (PGK promoter), E.coli neo, mouse Pgap3 genomic DNA |
Research application | Cell Biology Research Cre/loxP system FLP/frt system |
Specific Term and Conditions | The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again. |
Depositor | Taroh Kinoshita (Osaka University) |
Strain Status | Live mice Frozen embryos Frozen sperm |
Strain Availability | Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) Live mouse (3 to 6 months) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
---|
No Data |