Strain Data Sheet

RBRC06210

Strain Information

Image
BRC No.RBRC06210
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Pgap3<tm1>
Former Common namePgap3 floxed, B6-Pgap3 floxed
H-2 Haplotype
ES Cell lineEGR-G01 [129S2 x C57BL/6NCr-Tg(CAG/Acr-EGFP)]
Background strain
Appearance
Strain developmentDeveloped by Yetao Wang and Taroh Kinoshita, Research Institute for Microbial Diseases, Osaka University in 2007. EGRG01 ES cells derived from (129S2 x C57BL/6CrSlc)F1-Tg(GFP) were used for the generation of floxed mice. The mutant mice were backcrossed to C57BL/6J over 10 times.
Strain descriptionPgap3 gene floxed mice. Exon 7 and 8 were flanked by loxP sites. A FRT flanked neo cassette was inserted after the exon 8.
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJcl]
ReferencesBiochem. Biophys. Res. Commun., 417, 1235-1241(2012). 22227195
J. Biol. Chem., 88, 25490-25499 (2013). 23864655

Health Report

Examination Date / Room / Rack2024/01/09Room:3-BRack:E
2023/10/10Room:3-BRack:E

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Pgap3post-GPI attachment to proteins 311Pgap3<tm1Ymra>targeted mutation 1, Yoshiko Murakami

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site11Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site11Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP11loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP11loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)11mouse phosphoglycerate kinase promoter (PGK promoter)

Ordering Information

Donor DNAPhage P1 loxP sites, yeast flippase target sites, mouse phosphoglycerate kinase promoter (PGK promoter), E.coli neo, mouse Pgap3 genomic DNA
Research applicationCell Biology Research
Cre/loxP system
FLP/frt system
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
DepositorTaroh Kinoshita (Osaka University)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Live mouse (3 to 6 months)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data