Strain Data Sheet
ANIMAL SEARCH SYSTEM
Data update: Jun 20, 2019
Health Report   PCR Protocol   Thawing Method
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RBRC No. RBRC06211  
Type Targeted MutationCartagena  
Species Mus musculus  
Strain name B6.129-Piga<tm1>  
Former Common name Piga floxed, B6-Piga floxed  
H-2 Haplotype No Data  
ES cell line R1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]  
Background strain No Data  
Appearance
 1 Appearance No Data  
Genotype No Data  
Strain development Developed by Junji Takeda and Taroh Kinoshita, Research Institute for Microbial Diseases, Osaka University in 1997. R1 ES cells derived were used for the generation of floxed mice. The mutant mice were backcrossed to C57BL/6J over 10 times.  
Strain description Piga gene floxed mice. A loxP site was inserted between exons 5 and 6, and a loxP flanked Tk-neo cassette was inserted after the exon 5.  
Colony maintenance Heterozygote, Hemizygote x Wild-type [C57BL/6JJcl]  
Health Report
Gene Details
Promoter No Data  
 1 Symbol Piga  
Symbol name phosphatidylinositol glycan anchor biosynthesis, class A  
Chromosome X  
Common name No Data  
Symbol description No Data  
 2 Symbol loxP  
Symbol name phage P1 loxP  
Chromosome X  
Common name No Data  
Symbol description No Data  
Promoter herpes simplex virus thymidine kinase promoter (HSV tk promoter)  
 3 Symbol neo  
Symbol name neomycin resistance gene (E. coli)  
Chromosome X  
Common name No Data  
Symbol description No Data  
References   
Research applications Cre/loxP system,
Hematological Research,
Mouse Models for Human Disease  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.  
Additional information
 1 No Data  
 2 Genotyping protocol <PCR>  
Depositor KINOSHITA, Taroh (Osaka university)  KINOSHITA, Taroh 
Strain Status /
Availability
(Expected delivery)

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