Strain Data Sheet

RBRC06213

Strain Information

Image
BRC No.RBRC06213
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Igf1<tm1.2Hko>
Former Common nameIGF1 Delta FRT/+ (GWAS006), B6-Igf1 floxed
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Haruhiko Koseki, RIKEN Center for Allergy and Immunology. B6JN/1 ES cells derived from C57BL/6 were used for the generation of floxed mice. Neo cassette was removed by crossing with ACTB-FLPe mice (C57BL/6 background). The mutant mice were crossed to C57BL/6J.
Strain descriptionIGF1 (Igf1) gene floxed mice. Exon 2 was flanked by loxP sites.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6JJcl]
References

Health Report

Examination Date / Room / Rack2023/03/13Room:3-3Rack:C
2023/01/10Room:3-3Rack:C
2022/12/12Room:3-3Rack:C
2022/09/12Room:3-3Rack:C
2022/06/13Room:3-3Rack:C

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Igf1insulin-like growth factor 110Igf1<tm1.2Hko>targeted mutation 1.2, Haruhiko Koseki

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site10Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP10loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP10loxP

Ordering Information

Donor DNAphage P1 loxP sites, yeast FRT (flipase recombination target) site, mouse Igf1 genomic DNA
Research applicationCre/loxP system
FLP/frt system
Specific Term and ConditionsRECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorHaruhiko Koseki (RIKEN)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data