Strain Information | |
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Image | |
BRC No. | RBRC06215 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6.Cg-Plag1<tm1.2Hko> |
Former Common name | PLAG1 Delta FRT/+ (GWAS011), B6-Plag1 floxed |
H-2 Haplotype | |
ES Cell line | M1 [(C57BL/6 x 129)F1] |
Background strain | |
Appearance | |
Strain development | Developed by Haruhiko Koseki, RIKEN Center for Allergy and Immunology. M1 ES cells derived from (C57BL/6 x 129)F1 were used for the generation of floxed mice. Neo cassette was removed by crossing with ACTB-FLPe mice (C57BL/6 background). The mutant mice were crossed to C57BL/6J. |
Strain description | PLAG1 (Plag1) gene floxed mice. Exon 5 was flanked by loxP sites. |
Colony maintenance | Heterozygote x Wild-type [C57BL/6JJcl] |
References |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site4Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP4loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP4loxP |
Ordering Information | |
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Donor DNA | mouse Plag1 genomic DNA, phage P1 loxP, yeast FRT (flipase recombination target) site, human Beta-actin promoter, yeast flippase recombinase |
Research application | Cre/loxP system FLP/frt system |
Specific Term and Conditions | RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. |
Depositor | Haruhiko Koseki (RIKEN) |
Strain Status | Frozen embryos |
Strain Availability | Recovery and QC required prior to distribution |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |