Strain Data Sheet
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Data update: Jun 20, 2019
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RBRC No. RBRC06247  
Type Targeted MutationCartagena  
Species Mus musculus  
Strain name B6.Cg-Gab2<tm5.1Thir>  
Former Common name Gab2 Y441F/Y465F/Y573F/Y603F/Y632F KI, Gab2 deltaSHP2/deltaPI3K KI, B6-Gab2/dSHP2/dPI3K K  
H-2 Haplotype No Data  
ES cell line M1 [(C57BL/6 x 129)F1]  
Background strain No Data  
Appearance
 1 Appearance No Data  
Genotype No Data  
Strain development Developed by Keigo Nishida, RIKEN Center for Allergy and Immunology. M1 ES cells derived from (C57BL/6 x 129)F1 were used for the generation of knockout mice. A floxed neo cassette was removed by crossing with B6-CAG-cre mice. C57BL/6 and 129 mixed background.  
Strain description Gab2 mutant gene knock-in mice. A cDNA encoding 89-665 amino acids was inserted into exon 2 of Gab2 gene. The cDNAs encoded wild-type (WT) (RBRC06244), Y603F/Y632F mutant abolishing the SHP2-binding domain (RBRC06245), Y441F/Y465F/Y573F mutant abolishing PI3K-binding domain (RBRC06246) and Y441F/Y465F/Y573F/Y603F/Y632F mutant abolishing the SHP2 and PI3K-binding domains (RBRC06247) mutant, respectively.  
Colony maintenance Homozygote x Homozygote [or Crossing to C57BL/6JJcl]  
Health Report No Data  
Gene Details
Promoter No Data  
 1 Symbol No Data  
Symbol name mouse Gab2 Y441F/Y465F/Y573F/Y603F/Y632F mutant cDNA encoding amino acid 89-665  
Chromosome 7  
Common name No Data  
Symbol description No Data  
 2 Symbol GH  
Symbol name Growth hormone polyA (Bovine)  
Chromosome 7  
Common name No Data  
Symbol description No Data  
 3 Symbol Gab2  
Symbol name growth factor receptor bound protein 2-associated protein 2  
Chromosome 7  
Common name No Data  
Symbol description No Data  
 4 Symbol loxP  
Symbol name phage P1 loxP  
Chromosome 7  
Common name No Data  
Symbol description No Data  
References   
Research applications Cell Biology Research,
Cre/loxP system  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
J. Immunol., 187, 932-941 (2011).  
Additional information
 1 No Data  
Depositor Nishida, Keigo (RIKEN Center for Integrative Medical Sciences (IMS))  Nishida, Keigo 
Strain Status /
Availability
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