Strain Data Sheet

RBRC06251

Strain Information

Image
BRC No.RBRC06251
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Ret<tm1Mat> Ptch1<tm1Mps>
Former Common nameRet YF; Ptch1 KO, B6-RetYF KI/Ptch1 KO
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentRet Y1062F knock-in: Developed by Masahide Takahashi, Nagoya University Graduate School of Medicine. Ptch1 KO: Developed by Matthew Scott, Stanford University School of Medicine.
Strain descriptionRet Y1062F knock-in and Ptch1 KO double mutant mice. The Ret Y1062F knock-in allele consists of two amino acid replacement (KLY<1062> to KIF<1062>) within exon 19 and a floxed PGK-neo cassette inserted into intron 19. Part of exon 1 and all of exon 2 of Ptch1 gene were replaced with a lacZ-neo cassette in the Ptch1 KO allele.
Colony maintenanceRet: Heterozygote x Wild-type; Ptch1: Heterozygote x Wild-type [or Crossing to C57BL/6JJmsSlc]
ReferencesMol. Cell Biol., 24, 8026-8036 (2004). 15340065
Science, 277, 1109-1113 (1997). 9262482
Proc. Natl. Acad. Sci. USA, 107(29), 13051-13056 (2010). 20616061

Health Report

Examination Date / Room / Rack2023/04/10Room:3-ARack:J
2023/01/10Room:3-ARack:J
2022/10/11Room:3-ARack:J
2022/07/11Room:3-ARack:J
2022/04/11Room:3-ARack:J

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Ptch1patched 113Ptch1<tm1Mps>targeted mutation 1, Matthew P Scott

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
lacZbeta-galactosidase (E. coli)13

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)13

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Retret proto-oncogene6Ret<tm1Mat>targeted mutation 1, Masahide Takahashi

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP6loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP6loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)6mouse phosphoglycerate kinase promoter (PGK promoter)

Ordering Information

Donor DNAphage P1 LoxP sites, Mus musclus phosphoglycerate kinase promoter (PGK promoter), Escherichia coli Neomycin resistance gene, mouse Ret genomic DNA, Escherichia coli LacZ gene, mouse Ptch1 genomic DNA
Research applicationCre/loxP system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorMasashi Kato (Chubu University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data