Strain Information | |
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Image | |
BRC No. | RBRC06293 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6.Cg-Ngly1<tm1.1Tasuz> |
Former Common name | PNGase KO, B6-Ngly1 KO |
H-2 Haplotype | |
ES Cell line | KY1.1[(C57BL/6J x 129S6/SvEvTac)F1] |
Background strain | |
Appearance | |
Strain development | Developed by Tadashi Suzuki, Global Research Cluster, RIKEN. KY1.1 ES cells derived from (C57BL/6J x 129S6/SvEvTac)F1 were used. Exons 11 and 12 of Ngly gene were removed by crossing with Cre mice (B6). The mutant mice were backcrosses to C57BL/6 over 13 times. |
Strain description | Ngly gene knockout mice. Exons 11 and 12 were removed. Homozygous mutant mice are lethal. |
Colony maintenance | Heterozygote x Wild-type [C57BL/6J(Crj)] |
References | Proc. Natl. Acad. Sci. USA, 112(5) 1398-403 (2015). 25605922 PLoS Genet., 13(4):e1006696 (2017). 28426790 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site14Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP14loxP |
Ordering Information | |
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Donor DNA | Phage P1 loxP site, yeast FRT (flipase recombination target) site, mouse Ngly1 genomic DNA |
Research application | Cre/loxP system FLP/frt system Mouse Models for Human Disease Neurobiology Research |
Specific Term and Conditions | Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. PLoS Genet., 13(4):e1006696 (2017). |
Depositor | Tadashi Suzuki (RIKEN) |
Strain Status | Frozen sperm |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |