Strain Information | |
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Image | |
BRC No. | RBRC06294 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6;129-Aqp7<tm1Tfun> |
Former Common name | Aqp7 KO, B6-Aqp7 KO |
H-2 Haplotype | |
ES Cell line | |
Background strain | |
Appearance | |
Strain development | Developed by Norikazu Maeda, Tohru Funahashi and Iichiro Shimomura. 129 derived ES cells were used. The mutant mice were crossed to C57BL/6 over 5 times. |
Strain description | Aquaporin 7 (Aqp7) gene knockout mice. Exon 1-3 containing the translation start site was replaced with a neo cassette. |
Colony maintenance | Homozygote x Homozygote [or Crossing to C57BL/6NCrlCrlj] |
References | Proc. Natl. Acad. Sci. USA, 102, 10993-10998 (2005). 16009937 Proc. Natl. Acad. Sci. USA, 101, 17801-17806 (2004). 15591341 Cardiovasc. Res., 83, 34-41 (2009). 19297367 |
Health Report | |
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Examination Date / Room / Rack | 2023/07/24Room:3-CRack:G 2023/04/24Room:3-CRack:G 2023/01/23Room:3-CRack:G 2022/10/25Room:3-CRack:G 2022/07/25Room:3-CRack:G |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP4loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP4loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)4mouse phosphoglycerate kinase promoter (PGK promoter) |
Ordering Information | |
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Donor DNA | phage P1 loxP sites, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli Neomycin resistance gene, mouse Pgk1 polyA, mouse Aqp7 genomic DNA |
Research application | Cardiovascular Research Cre/loxP system Metabolism Research covid19_progression |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc. Nat.l Acad. Sci. USA, 101, 17801-17806 (2004).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. |
Depositor | Iichiro Shimomura (Osaka University) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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Ishihama S, Yoshida S, Yoshida T, Mori Y, Ouchi N, Eguchi S, Sakaguchi T, Tsuda T, Kato K, Shimizu Y, Ohashi K, Okumura T, Bando YK, Yagyu H, Wettschureck N, Kubota N, Offermanns S, Kadowaki T, Murohara T, Takefuji M. LPL/AQP7/GPD2 promotes glycerol metabolism under hypoxia and prevents cardiac dysfunction during ischemia. FASEB J 35(12) e22048(2021) 34807469 Fujii M, Bessho R, Akimoto T, Ishii Y. Cardioprotective effect of St. Thomas' Hospital No. 2 solution against age-related changes in aquaporin-7-deficient mice. Gen Thorac Cardiovasc Surg (2023) 37691043 Fujii M, Ota K, Bessho R. Cardioprotective effect of hyperkalemic cardioplegia in an aquaporin 7-deficient murine heart. Gen Thorac Cardiovasc Surg 68(6) 578-584(2020) 31707553 Raje V, Ahern KW, Martinez BA, Howell NL, Oenarto V, Granade ME, Kim JW, Tundup S, Bottermann K, Gödecke A, Keller SR, Kadl A, Bland ML, Harris TE. Adipocyte lipolysis drives acute stress-induced insulin resistance. Sci Rep 10(1) 18166(2020) 33097799 |