Strain Data Sheet
ANIMAL SEARCH SYSTEM
Data update: Jun 20, 2019
Health Report   PCR Protocol   Thawing Method
embryos / sperm >
  Mouse Marking
Figure
  Order
RBRC No. RBRC06303  
Type Targeted MutationCartagena  
Species Mus musculus  
Strain name B6.Cg-Malat1<tm1.1Shna>/ShnaRbrc  
Former Common name Malat1 KO, B6-Malat1 KO, NEAT2(lacZ)(Acc.No.CDB0837K)  
H-2 Haplotype No Data  
ES cell line TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]  
Background strain No Data  
Appearance
 1 Appearance No Data  
Genotype No Data  
Strain development Developed by Shinichi Nakagawa, RIKEN Advanced Science Institute. TT2 ES cells were used to generate the knockout mice. A FRT-flanked neo cassette was removed by crossing with FLP mice. The mutant mice were backcrossed to C57BL/6N.  
Strain description Malat1 ncRNA knock-out mice. Homozygous mutant mice are viable and fertile.  
Colony maintenance Homozygote x Homozygote [or Crossing to C57BL/6NCrSlc]  
Health Report
Gene Details
Promoter No Data  
 1 Symbol Frt  
Symbol name yeast FRT (flippase recombination target) site  
Chromosome 19  
Common name No Data  
Symbol description No Data  
 2 Symbol Malat1  
Symbol name metastasis associated lung adenocarcinoma transcript 1 (non-coding RNA)  
Chromosome 19  
Common name No Data  
Symbol description No Data  
 3 Symbol lacZ  
Symbol name beta-galactosidase (E. coli)  
Chromosome 19  
Common name No Data  
Symbol description No Data  
 4 Symbol lox71  
Symbol name mutant lox sequence  
Chromosome 19  
Common name No Data  
Symbol description No Data  
 5 Symbol loxP  
Symbol name phage P1 loxP  
Chromosome 19  
Common name No Data  
Symbol description No Data  
References   
Research applications Cell Biology Research,
Cre/loxP system,
FLP/frt system,
Fluorescent Proteins/lacZ System  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
(1) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the Acc.No.CDB0837K and Web. Site (http://www.cdb.riken.jp/arg/mutant%20mice%20list.html) is requested. (2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designed by the DEPOSITOR is requested (RNA. 2012 18(8):1487-99.). (3) Please inform the DEPOSITOR (mutant@cdb.riken.jp) about the paper when published.  
Additional information
 1 No Data  
 2 Genotyping protocol <PCR>  
Depositor RIKEN, CDB (RIKEN Center for Developmental Biology)  RIKEN, CDB 
Strain Status /
Availability
(Expected delivery)

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