Strain Information | |
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Image | |
BRC No. | RBRC06306 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | ICR;129(B6)-Mafg<tm1.1Jeng> |
Former Common name | MafG KO |
H-2 Haplotype | |
ES Cell line | R1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>] |
Background strain | |
Appearance | |
Strain development | Developed by University of Michigan, Douglas Engel and Masayuki Yamamoto, Institute of Basic Medical Sciences and Center for TARA, University of Tsukuba. R1 RS cells were used for generation of the mice. 129, C57BL/6 and ICR mixed background. A MC1-TK MC1-neo cassette of MafF KO (RBRC06305) and MafG KO (RBRC06306) mice was removed by crossing with Ayu1-cre mice. A PGK-neo cassette of MafK KO mice (RBRC06307) was removed by crossing with Ayu1-cre mice. |
Strain description | Small Maf protein genes knockout mice (MafF: RBRC06305, MafG: RBRC06306 and MafK: RBRC06307). Both MafF and MafK homozygous mutant mice show no obvious abnormality. MafG homozygous mutant mice exhibit impaired platelet formation accompanied by megakaryocyte proliferation, as well as mild behavioral abnormalities. MafF, MafG and MafK triple homozygous mutant mice are embryonic lethal (around E11.5). |
Colony maintenance | Heterozygote x Wild-type [or Crossing to Jcl:ICR] |
References | Genes Dev., 12, 2164-2174 (1998). 9679061 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Mafgv-maf musculoaponeurotic fibrosarcoma oncogene family, protein G (avian)11Mafg<tm1.1Jeng>targeted mutation 1.1, James Douglas Engel Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter lacZbeta-galactosidase (E. coli)11 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP11loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter nlsSV40 large T antigen nuclear localization signal (NLS)11nls |
Ordering Information | |
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Donor DNA | SV40 large T antigen nuclear localization signal (NLS), E. coli lacZ, phage P1 loxP, mouse Mafg genomic DNA |
Research application | Cre/loxP system Fluorescent Proteins/lacZ System Neurobiology Research |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Genes Dev., 12, 2164-2174 (1998). |
Depositor | Masayuki Yamamoto (Tohoku University) |
Strain Status | Frozen sperm |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Genotyping protocol -PCR- Necessary documents for ordering:
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BRC mice in Publications |
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No Data |