Strain Data Sheet
ANIMAL SEARCH SYSTEM
Data update: Aug 20, 2019
Health Report   PCR Protocol   Thawing Method
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RBRC No. RBRC06313  
Type Targeted MutationCartagena  
Species Mus musculus  
Strain name B6.129-Sparc<tm1Hwe>  
Former Common name B6-Sparc KO  
H-2 Haplotype No Data  
ES cell line CCE/EK.CCE [129S/SvEv-Gpi1]  
Background strain No Data  
Appearance
 1 Appearance No Data  
Genotype No Data  
Strain development Developed by Chin Chen Howem The Wistar Institute. CCB ES cells were used to generate the knockout mice. The mutant mice were maintained by Yasumasa Kato, Ohu university School of Dentistry through Helene Sga, Benaroya Research Institute at Virginia. C57BL/6 congenic.  
Strain description Sparc (osteonectin, BM-40) gene knockout mice. Exon 4 of Sparc gene was replaced with MC1-neo and Pol2-neo tandem cassettes. Homozygous mutant mice exhibit cataracts, abnormal skeletal morphology and osteopenia (viable and fertile).  
Colony maintenance Homozygote x Homozygote [or Crossing to C57BL/6JJcl]  
Health Report
Gene Details
Promoter No Data  
 1 Symbol GH  
Symbol name Growth hormone polyA (Bovine)  
Chromosome 11  
Common name No Data  
Symbol description No Data  
 2 Symbol Sparc  
Symbol name secreted acidic cysteine rich glycoprotein  
Chromosome 11  
Common name No Data  
Symbol description No Data  
Promoter herpes simplex virus thymidine kinase promoter (HSV tk promoter)  
 3 Symbol neo  
Symbol name neomycin resistance gene (E. coli)  
Chromosome 11  
Common name No Data  
Symbol description No Data  
Promoter mouse RNA polymerase II promoter  
 4 Symbol neo  
Symbol name neomycin resistance gene (E. coli)  
Chromosome 11  
Common name No Data  
Symbol description No Data  
References
  

Research applications Cancer Research,
Cell Biology Research,
Developmental Biology Research,
Hematological Research  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Invest. Ophthalmol. Vis. Sci., 39, 2674-2680 (1998 ).  
Additional information
 1 No Data  
 2 Genotyping protocol <PCR>  
Depositor Kato, Yasumasa (Kanagawa Dental College)  Kato, Yasumasa 
Strain Status /
Availability
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