Strain Data Sheet
ANIMAL SEARCH SYSTEM
Data update: Jul 19, 2019
Health Report   PCR Protocol   Thawing Method
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  Mouse Marking
Figure
  Order
RBRC No. RBRC06315  
Type Targeted MutationCartagena  
Species Mus musculus  
Strain name MF1.129-Sparc<tm1Cam>  
Former Common name MF1-Sparc KO  
H-2 Haplotype No Data  
ES cell line CCB [129S/SvEv-Gpi1]  
Background strain No Data  
Appearance
 1 Appearance No Data  
Genotype No Data  
Strain development Developed by William H. Colledge, University of Cambridge. CCB ES cells were used. Maintained by Yasumasa Kato, Ohu university School of Dentistry. C3H congenic (over N9 generation) (RBRC06314), MF1 mixed background (RBRC06315).  
Strain description Sparc (osteonectin, BM-40) gene knockout mice. Exon 6 of Sparc gene was replaced with a SA-lacZ/PGK-neo cassette. Homozygous mutant mice exhibit cataracts.  
Colony maintenance Homozygote x Homozygote [or Crossing to HsdOla:MF1]  
Health Report
Gene Details
Promoter No Data  
 1 Symbol GH  
Symbol name Growth hormone polyA (Bovine)  
Chromosome 11  
Common name No Data  
Symbol description No Data  
 2 Symbol SA  
Symbol name Splice acceptor (adenovirus)  
Chromosome 11  
Common name No Data  
Symbol description No Data  
 3 Symbol Sparc  
Symbol name secreted acidic cysteine rich glycoprotein  
Chromosome 11  
Common name No Data  
Symbol description No Data  
 4 Symbol lacZ  
Symbol name beta-galactosidase (E. coli)  
Chromosome 11  
Common name No Data  
Symbol description No Data  
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)  
 5 Symbol neo  
Symbol name neomycin resistance gene (E. coli)  
Chromosome 11  
Common name No Data  
Symbol description No Data  
References
  

Research applications Cancer Research,
Cell Biology Research,
Developmental Biology Research,
Fluorescent Proteins/lacZ System,
Hematological Research  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
EMBO J., 17, 1860-1870 (1998).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.  
Additional information
 1 No Data  
 2 Genotyping protocol <PCR>  
Depositor Kato, Yasumasa (Kanagawa Dental College)  Kato, Yasumasa 
Strain Status /
Availability
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