Strain Information | |
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Image | |
BRC No. | RBRC06315 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | MF1.129-Sparc<tm1Cam> |
Former Common name | MF1-Sparc KO |
H-2 Haplotype | |
ES Cell line | CCB [129S/SvEv-Gpi1<c>] |
Background strain | |
Appearance | |
Strain development | Developed by William H. Colledge, University of Cambridge. CCB ES cells were used. Maintained by Yasumasa Kato, Ohu university School of Dentistry. C3H congenic (over N9 generation) (RBRC06314), MF1 mixed background (RBRC06315). |
Strain description | Sparc (osteonectin, BM-40) gene knockout mice. Exon 6 of Sparc gene was replaced with a SA-lacZ/PGK-neo cassette. Homozygous mutant mice exhibit cataracts. |
Colony maintenance | Homozygote x Homozygote [or Crossing to HsdOla:MF1] |
References | EMBO J., 17, 1860-1870 (1998). 9524110 Int. J. Oncol., 27, 759-768 (2005). 16077926 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Sparcsecreted acidic cysteine rich glycoprotein11Sparc<tm1Cam>targeted mutation 1, University of Cambridge Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter GHGrowth hormone polyA (Bovine)11GH Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter SASplice acceptor (adenovirus)11SA Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter lacZbeta-galactosidase (E. coli)11 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)11mouse phosphoglycerate kinase promoter (PGK promoter) |
Ordering Information | |
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Donor DNA | mouse Sparc genomic DNA, adenovirus splice acceptor, E. coli lacZ, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neomycine resistance gene, bovine growth hormone gene polyadenylation signal |
Research application | Cancer Research Cell Biology Research Developmental Biology Research Fluorescent Proteins/lacZ System Hematological Research |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. EMBO J., 17, 1860-1870 (1998).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. |
Depositor | Yasumasa Kato (Kanagawa Dental College) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved embryos (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |