Strain Information | |
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Image | |
BRC No. | RBRC06327 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6;129-Ffar3<tm1Reh> |
Former Common name | Gpr41 KO, B6-Ffar3 KO |
H-2 Haplotype | |
ES Cell line | SM1[129S6/SvEvTac] |
Background strain | |
Appearance | |
Strain development | Developed by Masashi Yanagisawa, The University of Texas Southwestern Medical Center. SM1 ES cells derived from 129S6/SvEvTac mice were used to generate the mutant mice. The mice were crossed to C57BL/6. |
Strain description | The Gpr41 (Ffar3) exon coding region was replaced with a nLacZ and floxed neo cassette. |
Colony maintenance | Homozygote x Homozygote [or Crossing to C57BL/6J(Crjl)] |
References | Proc. Natl. Acad. Sci. USA, 105, 16767-1672 (2008). 18931303 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter lacZbeta-galactosidase (E. coli)7 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP7loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP7loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)7mouse phosphoglycerate kinase promoter (PGK promoter) Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter nlsSV40 large T antigen nuclear localization signal (NLS)7nls |
Ordering Information | |
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Donor DNA | SV40 large T antigen nuclear localization signal (NLS), E. coli lacZ, phage P1 loxP sites, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli Neomycin resistance gene, mouse Ffar3 genomic DNA |
Research application | Cre/loxP system Fluorescent Proteins/lacZ System |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc. Natl. Acad. Sci. U S A. 105, 16767-1672 (2008).Depositor limits the use of the BIOLOGICAL RESOURCE to not-for-profit institutions. Depositor limits the use of the BIOLOGICAL RESOURCE to academic purposes. For-profit institutions and recipients who intend to use BIOLOGICAL RESOURCE for commercial research purposes shall acquire prior written consent from the Depositor. Recipient shall acquire prior written consent from the Depositor prior to filing an application for patent, or intellectual property or other rights based on the results of research using the BIOLOGICAL RESOURCE. |
Depositor | Masashi Yanagisawa (University of Texas Southwestern Medical Center at Dallas) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |