Strain Data Sheet

RBRC06331

Strain Information

Image
BRC No.RBRC06331
TypeGene TrapCartagena
SpeciesMus musculus
Strain nameB6;129-Dtx4<Gt(431E3)Cmhd>
Former Common nameDtx4 KO, B6-Dtx4 Gt
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strain
Appearance
Strain developmentDeveloped by Naifang Lu, Athanasia Spandidos and Brian Seed, Massachusetts General Hospital. The gene trapped mice were generated by using a CMHD-GT_431-E3-3 ES clone from Canadian Mouse Mutant Repository. C57BL/6 and 129 mixed background.
Strain descriptionDtx4 gene trapped mice (originated from Canadian Mouse Mutant Repository, Vector: pNMDi4, CMHD-GT_431-E3-3 clone. Homozygous mutant mice show no obvious abnormality. Under investigation (details unknown).
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6NTac]
References

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Dtx4deltex 4 homolog (Drosophila)19Dtx4<Gt(431E3)Cmhd>gene trap 431E3, Centre for Modeling Human Disease

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
GHGrowth hormone polyA (Bovine)19GH

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Hprtmouse Hprt splice donor (SD)19Hprt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SAhuman adenovirus type 3/5 SA (splice acceptor)19SA

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
VenusYellow Fluorescent Protein (Aequorea victoria)19Venus

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP19loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP19loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)19mouse phosphoglycerate kinase promoter (PGK promoter)

Ordering Information

Donor DNAHuman adenovirus type 3/5 SA (splice acceptor), flexible linker (FL), jellyfish Venus, P1 phage loxP, Eukaryotic translation initiation factor 3 subunit 7, BGH polyA, mouse PGK promoter, E. coli neomycin resistance gene, mouse Hprt SD (splice donor)
Research applicationCre/loxP system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsIn publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE containing Venus, an acknowledgment to Dr. Atsushi Miyawaki and a citation of the following literature(s) designated by the DEPOSITOR are requested.
Nat. Biotechnol., 20, 87-90 (2002).
DepositorBrian Seed (Massachusetts General Hospital)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

BRC mice in Publications

No Data