Strain Information | |
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Image | |
BRC No. | RBRC06350 |
Type | Gene Trap |
Species | Mus musculus |
Strain name | B6.129S2-Asf1b<Gt(ROSABetageo)437Muv>/Skmz |
Former Common name | B6-CIA-II, B6-Asf1b Gt |
H-2 Haplotype | |
ES Cell line | |
Background strain | |
Appearance | |
Strain development | Developed by Kazuhisa Sekimizu, Graduate School of Pharmaceutical Sciences in 2007. CIA-II (RBRC06350): Originated from Asf1b<Gt(ROSABetageo)437Muv> gene trapped mice (EMMA ID:01154). Backcrossed to C57BL/6. CIA-I (RBRC06351 and RBRC06352): Generated by using Asf1a<Gt(S7-4B1)Sor> gene trapped ES cells (MMRRC #13934-USD/S7-4B1). The mutant mice were backcrossed to ICR and C57BL/6 mice, respectively. |
Strain description | CIA-II(Asf1b) gene trapped mice (B6 background)(RBRC06350), CIA-I (Asf1a) gene trapped mice (ICR mixed background: RBRC06351 and B6 background: RBRC06352). Homozygous and heterozygous mutant mice for CIA-II gene show no obvious abnormality. Homozygous mutant mice for CIA-I of ICR mixed and C57BL/6 background were perinatal and embryonic (around E12.5) lethal, respectively. |
Colony maintenance | Homozygote x Homozygote [or Crossing to C57BL/6JJcl] |
References | Genes Dev., 5, 1513-1523 (1991). 1653172 Genes Dev., 14, 1332-1342 (2000). 10837026 Nat. Genet., 36, 304-312 (2004). 14981515 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Asf1bASF1 anti-silencing function 1 homolog B (S. cerevisiae)8Asf1b<Gt(ROSABetageo)437Muv>gene trap 437, Muriel Vernet Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter GHGrowth hormone polyA (Bovine)8GH Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter LTRretrovirus LTR (viral long terminal repeat)8LTR Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter SASplice acceptor (adenovirus)8SA Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter lacZbeta-galactosidase (E. coli)8 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)8 |
Ordering Information | |
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Donor DNA | adenovirus splice acceptor (SA), E. coli beta-galactosidase, E. coli Neomycin resistance gene, bovine growth hormone polyadenylation signal, retrovial LTR, mouse Asf1a genomic DNA |
Research application | Cancer Research Fluorescent Proteins/lacZ System |
Specific Term and Conditions | Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (will be announced.) |
Depositor | Kazuhisa Sekimizu (The University of Tokyo) |
Strain Status | Frozen sperm |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |