Strain Information | |
---|---|
Image | |
BRC No. | RBRC06352 |
Type | Gene Trap |
Species | Mus musculus |
Strain name | B6.129S4-Asf1a<Gt(S7-4B1)Sor>/Skmz |
Former Common name | B6-CIA-I, B6-Asf1a Gt |
H-2 Haplotype | |
ES Cell line | |
Background strain | C57BL/6JJcl |
Appearance | |
Strain development | Developed by Kazuhisa Sekimizu, Graduate School of Pharmaceutical Sciences in 2007. CIA-II (RBRC06350): Originated from Asf1b<Gt(ROSABetageo)437Muv> gene trapped mice (EMMA ID:01154). Backcrossed to C57BL/6. CIA-I (RBRC06351 and RBRC06352): Generated by using Asf1a<Gt(S7-4B1)Sor> gene trapped ES cells (MMRRC #13934-USD/S7-4B1). The mutant mice were backcrossed to ICR and C57BL/6 mice, respectively. |
Strain description | CIA-II(Asf1b) gene trapped mice (B6 background)(RBRC06350), CIA-I (Asf1a) gene trapped mice (ICR mixed background: RBRC06351 and B6 background: RBRC06352). Homozygous and heterozygous mutant mice for CIA-II gene show no obvious abnormality. Homozygous mutant mice for CIA-I of ICR mixed and C57BL/6 background were perinatal and embryonic (around E12.5) lethal, respectively. |
Colony maintenance | Heterozygote x Wild-type [C57BL/6JJcl] |
References | Nat. Genet., 36, 304-312 (2004). 14981515 Genes Dev., 14, 1332-1342 (2000). 10837026 Genes Dev., 5, 1513-1523 (1991). 1653172 |
Health Report | |
---|---|
Examination Date / Room / Rack |
Gene | |
---|---|
Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Asf1aanti-silencing function 1A histone chaperone10Asf1a<Gt(S7-4B1)Sor>gene trap S7-4B1, Philippe Soriano Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site10Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site10Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter GHGrowth hormone polyA (Bovine)10GH Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Hyghygromycin phasphotransferase (E. coli)10Hygmouse phosphoglycerate kinase promoter (PGK promoter) Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter LTRretrovirus LTR (viral long terminal repeat)10LTR Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter SASplice acceptor (adenovirus)10SA Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter SDadenovirus splice donor (SD)10SD Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter lacZbeta-galactosidase (E. coli)10 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)10 |
Ordering Information | |
---|---|
Donor DNA | adenovirus splice acceptor (SA), E. coli beta-galactosidase, E. coli Neomycin resistance gene, bovine growth hormone polyadenylation signal, yeast Frt sites, mouse phosphoglycerate kinase promoter, E. coli hygromycin resistance gene, adenovirus splice donor (SD), retroviral LTR, mouse Asf1b genomic DNA |
Research application | Developmental Biology Research FLP/frt system Fluorescent Proteins/lacZ System |
Specific Term and Conditions | Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (will be announced.) The use of the BIOLOGICAL RESOURCE is strictly limited to academic, non-profit research institutes for academic non-profit research purposes. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, a mention of the Sanger Institute Gene Trap Resource as the source of the original allele are requested. |
Depositor | Kazuhisa Sekimizu (The University of Tokyo) |
Strain Status | Frozen sperm |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
---|
No Data |