Strain Data Sheet

RBRC06386

Strain Information

Image
BRC No.RBRC06386
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6.Cg-Tg(Nepro-venus)1Tets
Former Common nameNepro-venus Tg#1, B6-Nepro-venus Tg#1
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Yuko Muroyama and Tetsuichiro Saito, Graduate School of Medicine, Chiba University in 2013. The transgene was injected into the pronuclei of B6D2F1 fertilized eggs. The mice were crossed to C57BL/6.
Strain descriptionNepro-venus transgenic mice having a venus gene under the control of the Nepro 5’ genomic region. Tg mice are viable and fertile.
Colony maintenanceCarrier x Noncarrier [C57BL/6JJcl]
ReferencesDevelopment, 136, 3889-3893 (2009). 19906856

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
VenusYellow Fluorescent Protein (Aequorea victoria)UNVenusmouse Nepro promoter

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 Intron and polyA signalUN

Ordering Information

Donor DNAmouse Nepro genomic DNA, containing promoter and enhancer, Aequorea victoria Venus gene, SV40 Intron and polyA signal
Research applicationFluorescent Proteins/lacZ System
Neurobiology Research
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Development, 136, 3889-3893 (2009).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE containing Venus, an acknowledgment to Dr. Atsushi Miyawaki and a citation of the following literature(s) designated by the DEPOSITOR are requested.
Nat. Biotechnol., 20, 87-90 (2002).
DepositorTetsuichiro Saito (Chiba University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data