Strain Data Sheet

RBRC06387

Strain Information

Image
BRC No.RBRC06387
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129P2-Ube4b<tm1Kei>
Former Common nameE4b KO, B6-Ube4b KO
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strain
Appearance
Strain developmentDeveloped by Keiichi Nakayama, Medical Institute of Bioregulation, Kyushu University. E14 ES cells were used. The mutant mice were crossed to C57BL/6J over 6 times.
Strain descriptionUbe4b (Ufd2a) gene knockout mice. Exons 23 to 27 were replaced with a PGK-neo (loxP-neo) cassette.
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJcl]
ReferencesMol. Cell Biol., 25, 10953-10964 (2005). 16314518

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Ube4bubiquitination factor E4B4Ube4b<tm1Kei>targeted mutation 1, Keiichi I Nakayama

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP4loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP4loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)4mouse phosphoglycerate kinase promoter (PGK promoter)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 polyA signal4

Ordering Information

Donor DNAE. coli Neomycin resistance gene, mouse phosphoglycerate kinase promoter, SV40 polyA signal, phage P1 loxP
Research applicationDevelopmental Biology Research
Neurobiology Research
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR.
DepositorKeiichi Nakayama (Kyushu University)
Strain Statusan icon for Frozen embryosFrozen embryos
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)
Lab HP

BRC mice in Publications

No Data