Strain Data Sheet
ANIMAL SEARCH SYSTEM
Data update: Jun 20, 2019
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RBRC No. RBRC06434  
Type Targeted MutationCartagena  
Species Mus musculus  
Strain name B6.Cg-Gt(ROSA)26Sor<tm1.1(CAG-SCAT1)Miur>  
Former Common name Rosa26-CAG-SCAT1, B6-ROSA26/SCAT1 KI  
H-2 Haplotype No Data  
ES cell line Bruce 4 [B6.Cg-Thy1]  
Background strain No Data  
Appearance
 1 Appearance No Data  
Genotype No Data  
Strain development Developed by Ting Liu, Yoshifumi Yamaguchi and Masayuki Miura, Graduate School of Pharmaceutical Sciences, University of Tokyo, and Katsuaki Hoshino and Tsuneyasu Kaisho, RIKEN Research Center for Allergy and Immunology. C57BL/6J and C57BL/6N mixed.  
Strain description CAG-CAST1 knock-in mice (delta STOP-neo type). A CAG-loxP-neo-loxP-SCAT1-pA cassette was inserted into the Gt(ROSA)26Sor locus, and then the floxed neo cassette was removed by crossing with CAG-Cre mice. Homozygous mice are viable (fertility is unknown).  
Colony maintenance Heterozygote x Wild-type [C57BL/6JJcl]  
Health Report No Data  
Gene Details
Promoter No Data  
 1 Symbol Gt(ROSA)26Sor  
Symbol name gene trap ROSA 26, Philippe Soriano  
Chromosome 6  
Common name No Data  
Symbol description No Data  
 2 Symbol Frt  
Symbol name yeast FRT (flippase recombination target) site  
Chromosome 6  
Common name No Data  
Symbol description No Data  
 3 Symbol GH  
Symbol name Growth hormone polyA (Bovine)  
Chromosome 6  
Common name No Data  
Symbol description No Data  
 4 Symbol Venus  
Symbol name Green Fluorescent Protein, F46L (Jellyfish)  
Chromosome 6  
Common name No Data  
Symbol description No Data  
 5 Symbol loxP  
Symbol name phage P1 loxP  
Chromosome 6  
Common name No Data  
Symbol description No Data  
Promoter CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)  
 6 Symbol ECFP  
Symbol name Enhanced Cyan Fluorescent Protein (Jellyfish)  
Chromosome 6  
Common name No Data  
Symbol description No Data  
References Liu et al., Single-cell imaging of caspase-1 dynamics reveals an all-or-none inflammasome signaling response Cell Reports, in press  
Research applications Cre/loxP system,
FLP/frt system,
Fluorescent Proteins/lacZ System  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Single-cell imaging of caspase-1 dynamics reveals an all-or-none inflammasome signaling response" Cell Reports, 8(4):974-982 (2014).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.  
Additional information
 1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.
Lab HP (Japanese)  
Depositor Miura, Masayuki (The University of Tokyo)  Miura, Masayuki 
Strain Status /
Availability
(Expected delivery)

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