Strain Data Sheet

RBRC06459

Strain Information

Image
BRC No.RBRC06459
TypeCRISPR/Cas9Cartagena
SpeciesMus musculus
Strain nameC57BL/6J-Tyr<em2Utr>
Former Common nameTyr G291T, B6-Tyr/G291T
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Fumihiro Sugiyama, Laboratory animal resource center, University of Tsukuba in 2014. Generated by injecting Cas9 and gRNA (GGGTGGATGACCGTGAGTCCTGG) expression plasmids into the C57BL/6J fertilized eggs. Off-target analysis was examined.
Strain descriptionTyr 5 bp (291-5) deletion mutant mice (RBRC06458). Tyr G291T mutant mice (RBRC06459). Homozygous mutant mice exhibit albino phenotype.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6J(Crlj)]
ReferencesMammalian Genome, 25, 327-334 (2014). 24879364

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Tyrtyrosinase7Tyr<em2Utr>endonuclease-mediated mutation 2, University of Tsukuba Laboratory Animal Resource Center

Ordering Information

Donor DNACRISPR/Cas9システムを用いて作成後、宿主への残存について未確認
Research application
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Simple generation of albino C57BL/6J mice with G291T mutation in the tyrosinase gene by the CRISPR/Cas9 system. Mammalian Genome, 25, 327-334 (2014).
DepositorFumihiro Sugiyama (University of Tsukuba)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data