Strain Data Sheet

RBRC06463

Strain Information

Image
BRC No.RBRC06463
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6-Prkcd<tm1.1Shb>
Former Common namePKCdelta KO, B6-Prkcd KO
H-2 Haplotype
ES Cell lineRENKA [C57BL/6NCrlCrlj]
Background strain
Appearance
Strain developmentDeveloped by Yuko Niino and Seiji Shiota, School of Medicine, Showa University and Tomoya Nakamachi, Graduate School of Science and Engineering for Education, University of Toyama. The mutant mice were generated from Prkcd<tm1Shb> mice (RBRC06462) by crossing with a Tg(Pgk2-cre)24Shb mice (PGK2-Cre transgenic mice).
Strain descriptionPrkcd gene knockout mice. Exon 7 of the Prkcd was deleted. Homozygous mutant mice are probably embryonic lethal. Under investigation.
Colony maintenanceHeterozygote x Wild-type [C57BL/6NCrlCrlj]
ReferencesPLoS One, 16(7):e0253912 (2021). 34197550

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Prkcdprotein kinase C, delta14Prkcd<tm1.1Shb>targeted mutation 1.1, Seiji Shiota

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP14loxP

Ordering Information

Donor DNAPharge P1 LoxP site, mouse PKCdelta genomic DNA
Research applicationCre/loxP system
Neurobiology Research
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. PLoS One, 16(7):e0253912 (2021).In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR/DEVELOPER (Drs. Seiji Shiota and Yuko Niino) is requested (will be announced). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR/DEVELOPER and its colleagues (Drs. Tomoya Nakamachi and Jun Watanabe) is requested. Before the first publication on the BIOLOGICAL RESOURCE by the DEPOSITOR/DEVELOPER, the RECIPIENT agrees to use the BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR/DEVELOPER, and then include them as co-authors in the publication.
DepositorSeiji Shiota (Showa University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data